嗜盐嗜碱菌中酸诱导紫色膜物种的研究。
Studies of an acid-induced species of purple membrane from Halobacterium halobium.
作者信息
Moore T A, Edgerton M E, Parr G, Greenwood C, Perham R N
出版信息
Biochem J. 1978 May 1;171(2):469-76. doi: 10.1042/bj1710469.
Abstract
A new spectral species of the purple membrane of Halobacterium halobium has been observed below pH 3.2. The formation of this new species is temperature-dependent and is favoured by increasing temperature up to the physiological range of the organism. The rate of formation at pH 3.0 and 22 degrees C is 7.9 x 10-3s-1. The spectral distribution and temperature-dependence of the new species suggest that it may be phototransiet O, stabilized by low pH. Flash-photolytic experiments in the pH range 7.2-2.7 show a pH-dependence corresponding to the static events and are consistent with a single protonation of bacteriorhodopsin below pH 3.22. These results can also be interpreted in terms of the stabilization of phototransient O at low pH. The temperature-dependence of the formation of the acid-induced species may reflect a relationship with the phase transition of the membrane.
摘要
在pH值低于3.2时，观察到了嗜盐菌紫色膜的一种新光谱物种。这种新物种的形成与温度有关，在达到该生物体的生理温度范围之前，温度升高有利于其形成。在pH值为3.0和22摄氏度时，形成速率为7.9×10⁻³ s⁻¹。新物种的光谱分布和温度依赖性表明，它可能是光瞬态O，在低pH值下得以稳定。在pH值范围为7.2 - 2.7的闪光光解实验显示，其pH依赖性与静态事件相对应，并且与在pH值低于3.22时细菌视紫红质的单质子化一致。这些结果也可以用光瞬态O在低pH值下的稳定作用来解释。酸诱导物种形成的温度依赖性可能反映了与膜相变的关系。
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