School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, Heilongjiang, China.
Department of Chemistry, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, China.
Anal Bioanal Chem. 2022 Jul;414(18):5087-5103. doi: 10.1007/s00216-021-03792-9. Epub 2022 Jan 3.
The chemical microenvironment in cells is extremely complex involving numerous species with drastically different concentration as well as temporal and spatial distribution. Especially, reactive species including ROS, RNS, RSS, and many inorganic ions are recognized to play very important roles. The concentrations of these species are constantly regulated and often depend on each other. While fluorescent probes have been widely used to study various cell biological processes, those simultaneously probing two species just emerged recently. In this review, we highlight dual-functional luminescent probes for the detection and fluorescence imaging of two and more analytes in cells. The content will cover small-molecule synthetic fluorescent probes, DNA nanoassemblies, and nanoparticle-based nanoprobes. The target analytes include reactive species such as ROS, RNS, and RSS and other ions and molecules such as H, Cl, Ca, Cu, and O.
细胞中的化学微环境极其复杂，涉及到许多浓度差异极大的物种，并且具有时间和空间分布的特点。特别是活性物质，包括 ROS、RNS、RSS 和许多无机离子，被认为具有非常重要的作用。这些物质的浓度不断受到调节，并且通常相互依赖。虽然荧光探针已被广泛用于研究各种细胞生物学过程，但最近才刚刚出现同时探测两种物质的探针。在本综述中，我们重点介绍了用于检测和荧光成像细胞中两种或更多分析物的双功能发光探针。内容将涵盖小分子合成荧光探针、DNA 纳米组装体和基于纳米颗粒的纳米探针。目标分析物包括 ROS、RNS 和 RSS 等活性物质以及 H、Cl、Ca、Cu 和 O 等其他离子和分子。
