Fu Cuicui, Wang Yi, Chen Gang, Yang Liyuan, Xu Shuping, Xu Weiqing
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun, The People's Republic of China.
Anal Chem. 2015 Oct 6;87(19):9555-8. doi: 10.1021/acs.analchem.5b02508. Epub 2015 Sep 8.
A surface-enhanced Raman scattering (SERS) measurement of 3,3',4,4'-tetrachlorobiphenyl (PCB77) with aptamer capturing in a microfluidic device was demonstrated. To construct the microfluidic chip, an ordered Ag nanocrown array was fabricated over a patterned polydimethylsiloxane (PDMS) that was achieved by replicating an anodic aluminum oxide (AAO) template. The patterned PDMS sheet was covered with another PDMS sheet having two input channel grooves to form a close chip. The Ag nanocrown array was used for the SERS enhancement area and the detection zone. PCB 77 aptamers were injected into one channel and the other allows for analytes (PCBs). The mercapto aptamers captured the targets in the mixed zone and were immobilized to the SERS detection zone via S-Ag bonds so as to further improve both the SERS sensitivity and selectivity of PCB77. Such an aptamer-based microfluidic chip realized a rapid SERS detection. The lowest detectable concentration of 1.0 × 10(-8) M was achieved for PCB77. This work demonstrates that the aptamer-modified SERS microfluidic sensor can be utilized for selective detections of organic pollutants in the environment.
展示了在微流控装置中利用适配体捕获对3,3',4,4'-四氯联苯（PCB77）进行表面增强拉曼散射（SERS）测量。为构建微流控芯片，在通过复制阳极氧化铝（AAO）模板实现的图案化聚二甲基硅氧烷（PDMS）上制备了有序的银纳米冠阵列。图案化的PDMS片材覆盖有另一个具有两个输入通道凹槽的PDMS片材，以形成封闭芯片。银纳米冠阵列用作SERS增强区域和检测区。将PCB 77适配体注入一个通道，另一个通道用于注入分析物（多氯联苯）。巯基化适配体在混合区捕获目标，并通过S-Ag键固定在SERS检测区，从而进一步提高了PCB77的SERS灵敏度和选择性。这种基于适配体的微流控芯片实现了快速SERS检测。对于PCB77，实现了1.0×10(-8) M的最低可检测浓度。这项工作表明，适配体修饰的SERS微流控传感器可用于环境中有机污染物的选择性检测。
