Gordon Anna, Basler Ryan, Bansept-Basler Pauline, Fanstone Vicky, Harinarayan Lakshmi, Grant Paul K, Birchmore Richard, Bayles Rosemary A, Boyd Lesley A, O'Sullivan Donal M
NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK.
Syngenta France, Route de Moyencourt, 78910, Orgerus, France.
Theor Appl Genet. 2015 Dec;128(12):2447-60. doi: 10.1007/s00122-015-2599-5. Epub 2015 Sep 4.
Four QTL conferring resistance to ergot were identified in the UK winter wheat varieties 'Robigus' and 'Solstice'. Two QTL co-located with semi-dwarfing alleles at the Rht loci Rht - 1B and Rht - 1D implicating a role of these DELLA proteins in infection success of Claviceps purpurea. The fungal pathogen Claviceps purpurea infects ovaries of a broad range of temperate grasses and cereals, including hexaploid wheat, causing a disease commonly known as ergot. Sclerotia produced in place of seed carry a cocktail of harmful alkaloid compounds that result in a range of symptoms in humans and animals, causing ergotism. Following a field assessment of C. purpurea infection in winter wheat, two varieties 'Robigus' and 'Solstice' were selected which consistently produced the largest differential effect on ergot sclerotia weights. They were crossed to produce a doubled haploid mapping population, and a marker map, consisting of 714 genetic loci and a total length of 2895 cM was produced. Four ergot reducing QTL were identified using both sclerotia weight and size as phenotypic parameters; QCp.niab.2A and QCp.niab.4B being detected in the wheat variety 'Robigus', and QCp.niab.6A and QCp.niab.4D in the variety 'Solstice'. The ergot resistance QTL QCp.niab.4B and QCp.niab.4D peaks mapped to the same markers as the known reduced height (Rht) loci on chromosomes 4B and 4D, Rht-B1 and Rht-D1, respectively. In both cases, the reduction in sclerotia weight and size was associated with the semi-dwarfing alleles, Rht-B1b from 'Robigus' and Rht-D1b from 'Solstice'. Two-dimensional, two-QTL scans identified significant additive interactions between QTL QCp.niab.4B and QCp.niab.4D, and between QCp.niab.2A and QCp.niab.4B when looking at sclerotia size, but not between QCp.niab.2A and QCp.niab.4D. The two plant height QTL, QPh.niab.4B and QPh.niab.4D, which mapped to the same locations as QCp.niab.4B and QCp.niab.4D, also displayed significant genetic interactions.
在英国冬小麦品种“Robigus”和“Solstice”中鉴定出了4个抗麦角病的数量性状基因座（QTL）。两个QTL与Rht基因座Rht - 1B和Rht - 1D上的半矮化等位基因共定位，这表明这些DELLA蛋白在麦角菌感染成功中发挥了作用。真菌病原体麦角菌可感染包括六倍体小麦在内的多种温带禾本科植物和谷类作物的子房，引发一种俗称麦角病的病害。取代种子产生的菌核含有多种有害生物碱化合物，会在人和动物身上引发一系列症状，导致麦角中毒。在对冬小麦中麦角菌感染情况进行田间评估后，选择了两个品种“Robigus”和“Solstice”，它们对麦角菌核重量始终产生最大的差异效应。将它们杂交产生了一个双单倍体作图群体，并构建了一个由714个遗传位点组成、总长度为2895厘摩的标记图谱。以菌核重量和大小作为表型参数，鉴定出了4个降低麦角病发病率的QTL；在小麦品种“Robigus”中检测到QCp.niab.2A和QCp.niab.4B，在品种“Solstice”中检测到QCp.niab.6A和QCp.niab.4D。抗麦角病QTL QCp.niab.4B和QCp.niab.4D的峰值分别与4B和4D染色体上已知的矮化（Rht）基因座Rht-B1和Rht-D1的标记相同。在这两种情况下，菌核重量和大小的降低都与半矮化等位基因有关，即来自“Robigus”的Rht-B1b和来自“Solstice”的Rht-D1b。二维双QTL扫描发现，在观察菌核大小时，QTL QCp.niab.4B和QCp.niab.4D之间以及QCp.niab.2A和QCp.niab.4B之间存在显著的加性相互作用，但QCp.niab.2A和QCp.niab.4D之间不存在。两个株高QTL，QPh.niab.4B和QPh.niab.4D，它们与QCp.niab.4B和QCp.niab.4D位于相同位置，也表现出显著的遗传相互作用。
