Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France.
Clermont Université, Université Blaise Pascal, Institut Pascal, TSA 60206, CS 60026, 63178 Aubière, France; CNRS, UMR 6602, IP, F-63171 Aubière, France.
Carbohydr Polym. 2015 Nov 20;133:213-20. doi: 10.1016/j.carbpol.2015.07.022. Epub 2015 Jul 13.
Nosemosis is one of the most common and widespread diseases of adult honeybees. The causative agents, Nosema apis and Nosema ceranae, belong to microsporidia some obligate intracellular eukaryotic parasites. In this study, 10 sulphated polysaccharides from algae were evaluated for their antimicrosporidian activity. They were first shown to inhibit the in vitro growth of the mammal microsporidian model, Encephalitozoon cuniculi. The most efficient polysaccharides were then tested for their ability to inhibit the growth of Nosema ceranae in experimentally-infected adult honeybees. Two polysaccharides extracted from Porphyridium spp. did not show any toxicity in honeybees and one of them allowed a decrease of both parasite load and mortality rate due to N. ceranae infection. A decrease in parasite abundance but not in mortality rate was also observed with an iota carrageenan. Our results are promising and suggest that algal sulphated polysaccharides could be used to prevent and/or control bee nosemosis.
微孢子虫病是成年蜜蜂最常见和广泛的疾病之一。病原体，蜂囊孢菌和中华蜜蜂囊孢菌，属于微孢子虫，一些专性细胞内真核寄生虫。在这项研究中，评估了 10 种来自藻类的硫酸化多糖的抗微孢子虫活性。它们首先被证明可以抑制哺乳动物微孢子虫模型，兔脑炎微孢子虫的体外生长。然后，最有效的多糖被测试其抑制在实验感染的成年蜜蜂中中华蜜蜂囊孢菌生长的能力。两种从紫菜属提取的多糖在蜜蜂中没有显示出任何毒性，其中一种多糖可以降低由于中华蜜蜂囊孢菌感染引起的寄生虫负荷和死亡率。iota 卡拉胶也观察到寄生虫丰度降低但死亡率没有降低。我们的结果很有希望，表明藻类硫酸化多糖可用于预防和/或控制蜜蜂微孢子虫病。
