Departament d'Òptica i Optometria, Universitat Politècnica de Catalunya·Barcelona Tech, Sant Nebridi 22, 08222-Terrassa, Barcelona, Spain.
Institute of Chemistry, University of Graz, NAWI Graz, Heinrichstrasse 28/III, 8010 Graz, Austria; ARENA - Association for Resource Efficient and Sustainable Technologies, Inffeldgasse 21b, 8010 Graz, Austria.
N Biotechnol. 2016 Jan 25;33(1):73-7. doi: 10.1016/j.nbt.2015.08.006. Epub 2015 Sep 5.
Bacillus megaterium uyuni S29, isolated from the Bolivian salt lake Uyuni, displays a high capability to produce poly[(R)-3-hydroxybutyrate] (PHB) in industrial culture media. In order to analyze the influence of salt on biomass formation and PHB production, cultivations at different NaCl concentrations were carried out according to the salinity conditions of the habitats of the strain's original isolation. In this preliminary report, the strain showed considerable adaptability to media of different salinity, obtaining the best results for both cellular growth and PHB production in media containing 45 g/L NaCl. The strain grew at 100 g/L NaCl and PHB production was observed even at high salt levels of 250 g/L without unwanted concurrent spore formation. Its tolerance to high salt concentrations together with auspicious PHB productivity makes this strain appealing not only for PHB production, but also for other biotechnological applications such as the treatment of salty wastewater; additional studies will be needed to further increase PHB productivity.
从玻利维亚盐湖乌尤尼分离得到的巨大芽孢杆菌 uyuni S29，在工业培养基中具有高产聚[(R)-3-羟基丁酸酯]（PHB）的能力。为了分析盐对生物量形成和 PHB 生产的影响，根据菌株原始分离栖息地的盐度条件，在不同 NaCl 浓度下进行了培养。在本初步报告中，该菌株对不同盐度的培养基表现出相当的适应性，在含有 45 g/L NaCl 的培养基中获得了最佳的细胞生长和 PHB 生产效果。该菌株可在 100 g/L NaCl 下生长，甚至在 250 g/L 的高盐水平下也观察到 PHB 生产，而不会产生不必要的伴生孢子。该菌株对高盐浓度的耐受性以及良好的 PHB 生产能力，不仅使其适合 PHB 生产，还适合其他生物技术应用，如处理含盐废水；需要进一步的研究来进一步提高 PHB 的生产能力。
