Yusibov Vidadi, Rabindran Shailaja, Commandeur Ulrich, Twyman Richard M, Fischer Rainer
Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware 19711, USA.
Drugs R D. 2006;7(4):203-17. doi: 10.2165/00126839-200607040-00001.
Plants viruses are versatile vectors that allow the rapid and convenient production of recombinant proteins in plants. Compared with production systems based on transgenic plants, viral vectors are easier to manipulate and recombinant proteins can be produced more quickly and in greater yields. Over the last few years, there has been much interest in the development of plant viruses as vectors for the production of vaccines, either as whole polypeptides or epitopes displayed on the surface of chimeric viral particles. Several viruses have been extensively developed for vaccine production, including tobacco mosaic virus, potato virus X and cowpea mosaic virus. Vaccine candidates have been produced against a range of human and animal diseases, and in many cases have shown immunogenic activity and protection in the face of disease challenge. In this review, we discuss the advantages of plant virus vectors, the development of different viruses as vector systems, and the immunological experiments that have demonstrated the principle of plant virus-derived vaccines.
植物病毒是多功能载体，可在植物中快速便捷地生产重组蛋白。与基于转基因植物的生产系统相比，病毒载体更易于操作，重组蛋白的生产速度更快、产量更高。在过去几年中，人们对开发作为疫苗生产载体的植物病毒兴趣浓厚，这些疫苗可以是完整多肽，也可以是嵌合病毒颗粒表面展示的表位。几种病毒已被广泛用于疫苗生产，包括烟草花叶病毒、马铃薯X病毒和豇豆花叶病毒。针对一系列人类和动物疾病的候选疫苗已经生产出来，并且在许多情况下，这些疫苗在面对疾病挑战时都显示出免疫原性活性和保护作用。在这篇综述中，我们讨论了植物病毒载体的优势、不同病毒作为载体系统的开发，以及证明植物病毒衍生疫苗原理的免疫学实验。
