Nosaki Shohei, Hoshikawa Ken, Ezura Hiroshi, Miura Kenji
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Tsukuba-Plant Innovation Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Plant Biotechnol (Tokyo). 2021 Sep 25;38(3):297-304. doi: 10.5511/plantbiotechnology.21.0610a.
The production of recombinant proteins is important in academic research to identify protein functions. Moreover, recombinant enzymes are used in the food and chemical industries, and high-quality proteins are required for diagnostic, therapeutic, and pharmaceutical applications. Though many recombinant proteins are produced by microbial or mammalian cell-based expression systems, plants have been promoted as alternative, cost-effective, scalable, safe, and sustainable expression systems. The development and improvement of transient expression systems have significantly reduced the period of protein production and increased the yield of recombinant proteins in plants. In this review, we consider the importance of plant-based expression systems for recombinant protein production and as genetic engineering tools.
重组蛋白的生产在学术研究中对于鉴定蛋白功能非常重要。此外，重组酶被用于食品和化学工业，并且诊断、治疗及制药应用需要高质量的蛋白质。尽管许多重组蛋白是通过基于微生物或哺乳动物细胞的表达系统生产的，但植物已被推广为替代的、具有成本效益的、可扩展的、安全的和可持续的表达系统。瞬时表达系统的发展和改进显著缩短了蛋白质生产周期并提高了植物中重组蛋白的产量。在这篇综述中，我们探讨了基于植物的表达系统对于重组蛋白生产以及作为基因工程工具的重要性。
