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Transient protein expression systems in plants and their applications.植物中的瞬时蛋白表达系统及其应用。
Plant Biotechnol (Tokyo). 2021 Sep 25;38(3):297-304. doi: 10.5511/plantbiotechnology.21.0610a.
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Production of pharmaceutical-grade recombinant aprotinin and a monoclonal antibody product using plant-based transient expression systems.使用植物性瞬时表达系统生产药用级重组抑肽酶和单克隆抗体产品。
Plant Biotechnol J. 2010 Jun;8(5):638-54. doi: 10.1111/j.1467-7652.2009.00495.x.
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Agroinfiltration as an Effective and Scalable Strategy of Gene Delivery for Production of Pharmaceutical Proteins.农杆菌浸润法作为一种用于生产药用蛋白质的有效且可扩展的基因传递策略。
Adv Tech Biol Med. 2013 Jun;1(1). doi: 10.4172/atbm.1000103.
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Plant molecular farming for the production of valuable proteins - Critical evaluation of achievements and future challenges.植物分子农业生产有价值的蛋白质——成就的批判性评价和未来的挑战。
J Plant Physiol. 2021 Mar-Apr;258-259:153359. doi: 10.1016/j.jplph.2020.153359. Epub 2021 Jan 5.
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Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production.植物分子农场：重组生物制药生产的可行平台。
Plants (Basel). 2020 Jul 4;9(7):842. doi: 10.3390/plants9070842.
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Viral vectors for production of recombinant proteins in plants.用于在植物中生产重组蛋白的病毒载体。
J Cell Physiol. 2008 Aug;216(2):366-77. doi: 10.1002/jcp.21423.
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Transient Protein Expression by Agroinfiltration in Lettuce.通过农杆菌浸润法在生菜中进行瞬时蛋白表达。
Methods Mol Biol. 2016;1385:55-67. doi: 10.1007/978-1-4939-3289-4_4.
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Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering.植物细胞囊：用于重组蛋白生产和代谢工程的可扩展平台。
Plant Biotechnol J. 2019 Aug;17(8):1560-1566. doi: 10.1111/pbi.13081. Epub 2019 Feb 14.
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Robust -Mediated Transient Expression in Two Duckweed Species (Lemnaceae) Directed by Non-replicating, Replicating, and Cell-to-Cell Spreading Vectors.由非复制型、复制型和细胞间传播载体介导的两种浮萍科植物中的稳健瞬时表达
Front Bioeng Biotechnol. 2021 Nov 4;9:5. doi: 10.3389/fbioe.2021.761073. eCollection 2021.
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Maximizing the Production of Recombinant Proteins in Plants: From Transcription to Protein Stability.最大限度地提高植物中重组蛋白的产量：从转录到蛋白质稳定性。
Int J Mol Sci. 2022 Nov 4;23(21):13516. doi: 10.3390/ijms232113516.
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Harnessing Transient Expression Systems with Plant Viral Vectors for the Production of Biopharmaceuticals in .利用植物病毒载体的瞬时表达系统在……中生产生物制药
Int J Mol Sci. 2025 Jun 9;26(12):5510. doi: 10.3390/ijms26125510.
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Biosimilars Targeting Pathogens: A Comprehensive Review of Their Role in Bacterial, Fungal, Parasitic, and Viral Infections.靶向病原体的生物类似药：对其在细菌、真菌、寄生虫和病毒感染中作用的全面综述
Pharmaceutics. 2025 Apr 28;17(5):581. doi: 10.3390/pharmaceutics17050581.
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Nanoscale warriors against viral invaders: a comprehensive review of Nanobodies as potential antiviral therapeutics.对抗病毒入侵者的纳米级战士：关于纳米抗体作为潜在抗病毒疗法的全面综述
MAbs. 2025 Dec;17(1):2486390. doi: 10.1080/19420862.2025.2486390. Epub 2025 Apr 9.
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From Plants to Plants: Plant-Derived Biological Polymers as Sustainable and Safe Nanocarriers for Direct Delivery of DNA to Plant Cells.从植物到植物：植物源生物聚合物作为将DNA直接递送至植物细胞的可持续且安全的纳米载体
Nano Lett. 2025 Apr 9;25(14):5572-5581. doi: 10.1021/acs.nanolett.4c05489. Epub 2025 Mar 26.
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Prokaryote- and Eukaryote-Based Expression Systems: Advances in Post-Pandemic Viral Antigen Production for Vaccines.基于原核生物和真核生物的表达系统：后疫情时代疫苗用病毒抗原生产的进展。
Int J Mol Sci. 2024 Nov 7;25(22):11979. doi: 10.3390/ijms252211979.
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Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana.在本氏烟中生产活性人艾杜糖-2-硫酸酯酶（IDS）酶。
Sci Rep. 2024 Oct 4;14(1):23066. doi: 10.1038/s41598-024-73778-x.
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Characterization of plant produced V antibodies against cobra venom toxins for antivenom therapy.用于抗蛇毒血清疗法的植物产生的抗眼镜蛇毒毒素V抗体的特性分析。
Biotechnol Rep (Amst). 2024 Apr 16;42:e00841. doi: 10.1016/j.btre.2024.e00841. eCollection 2024 Jun.
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Efficient Expression of Functionally Active Aflibercept with Designed N-glycans.具有设计的N-聚糖的功能活性阿柏西普的高效表达。
Antibodies (Basel). 2024 Apr 7;13(2):29. doi: 10.3390/antib13020029.
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Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System.利用筑波系统对不同大豆品种组织中瞬时蛋白表达效率的比较评估
Plants (Basel). 2024 Mar 16;13(6):858. doi: 10.3390/plants13060858.
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Neutralizing activity of anti-respiratory syncytial virus monoclonal antibody produced in .在中国生产的抗呼吸道合胞病毒单克隆抗体的中和活性。
Hum Vaccin Immunother. 2024 Dec 31;20(1):2327142. doi: 10.1080/21645515.2024.2327142. Epub 2024 Mar 20.
本文引用的文献
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Prevention of necrosis caused by transient expression in Nicotiana benthamiana by application of ascorbic acid.应用抗坏血酸预防烟草原生质体瞬时表达引起的细胞坏死。
Plant Physiol. 2021 Jun 11;186(2):832-835. doi: 10.1093/plphys/kiab102.
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Phase III: Randomized observer-blind trial to evaluate lot-to-lot consistency of a new plant-derived quadrivalent virus like particle influenza vaccine in adults 18-49 years of age.III 期：在 18-49 岁成人中评估一种新的植物源性四价病毒样颗粒流感疫苗批间一致性的随机观察者盲法试验。
Vaccine. 2021 Mar 5;39(10):1528-1533. doi: 10.1016/j.vaccine.2021.01.004. Epub 2021 Feb 10.
3
Efficient base editing in tomato using a highly expressed transient system.利用高效表达的瞬时系统在番茄中进行高效的碱基编辑。
Plant Cell Rep. 2021 Apr;40(4):667-676. doi: 10.1007/s00299-021-02662-z. Epub 2021 Feb 7.
4
The Pharmaceutical Industry in 2020. An Analysis of FDA Drug Approvals from the Perspective of Molecules.2020 年制药行业。从分子角度分析 FDA 药物批准。
Molecules. 2021 Jan 25;26(3):627. doi: 10.3390/molecules26030627.
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Plant-Based Vaccines: The Way Ahead?植物源疫苗：未来之路？
Viruses. 2020 Dec 22;13(1):5. doi: 10.3390/v13010005.
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Efficacy, immunogenicity, and safety of a plant-derived, quadrivalent, virus-like particle influenza vaccine in adults (18-64 years) and older adults (≥65 years): two multicentre, randomised phase 3 trials.植物源、四价、类病毒样颗粒流感疫苗在成人（18-64 岁）和老年人（≥65 岁）中的有效性、免疫原性和安全性：两项多中心、随机、3 期临床试验。
Lancet. 2020 Nov 7;396(10261):1491-1503. doi: 10.1016/S0140-6736(20)32014-6. Epub 2020 Oct 13.
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First human efficacy study of a plant-derived influenza vaccine.植物源流感疫苗的首次人体疗效研究。
Lancet. 2020 Nov 7;396(10261):1464-1465. doi: 10.1016/S0140-6736(20)32010-9. Epub 2020 Oct 13.
8
Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1.用植物源多聚体H5血凝素免疫可保护鸡抵抗高致病性禽流感病毒H5N1。
Vaccines (Basel). 2020 Oct 9;8(4):593. doi: 10.3390/vaccines8040593.
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Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production.植物分子农场：重组生物制药生产的可行平台。
Plants (Basel). 2020 Jul 4;9(7):842. doi: 10.3390/plants9070842.
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Radish sprouts as an efficient and rapidly available host for an agroinfiltration-based transient gene expression system.萝卜芽作为基于农杆菌浸润的瞬时基因表达系统的高效且快速可用的宿主。
Plant Biotechnol (Tokyo). 2020 Mar 25;37(1):89-92. doi: 10.5511/plantbiotechnology.19.1216a.
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