Wu Ping, Qin Guangxing, Qian Heying, Chen Tao, Guo Xijie
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Gene. 2016 Jan 10;575(2 Pt 1):264-9. doi: 10.1016/j.gene.2015.09.009. Epub 2015 Sep 5.
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a major viral pathogen of silkworm and remains a big challenge to the sericultural industry. Insulin-related peptide binding protein 2 (IBP2) gene, induced by BmCPV infection may play an important role in B. mori immune response. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the post-transcriptional level and play an important role in various processes, including immunity and antiviral response. In this study, we identified IBP2 as one of the targets for miR-278-3p by using luciferase reporter assay. Overexpression of miR-278-3p negatively regulates the expression of IBP2 in silkworm larvae and positively regulates the mRNA transcript level of BmCPV. Our results suggest that miR-278-3p may play an important role in BmCPV replication. It's the first report on bmo-miRNAs in response to BmCPV infection and could provide a new clue to explore the molecular mechanism of BmCPV infection and host immunity.
家蚕细胞质型多角体病毒（BmCPV）是家蚕的一种主要病毒病原体，仍然是养蚕业面临的一大挑战。受BmCPV感染诱导的胰岛素相关肽结合蛋白2（IBP2）基因可能在家蚕免疫反应中发挥重要作用。微小RNA（miRNA）是一类小的非编码RNA，它们在转录后水平调节基因表达，并在包括免疫和抗病毒反应在内的各种过程中发挥重要作用。在本研究中，我们通过荧光素酶报告基因检测确定IBP2是miR-278-3p的靶标之一。miR-278-3p的过表达在蚕幼虫中负向调节IBP2的表达，并正向调节BmCPV的mRNA转录水平。我们的结果表明，miR-278-3p可能在BmCPV复制中发挥重要作用。这是关于响应BmCPV感染的家蚕miRNA的首次报道，可为探索BmCPV感染和宿主免疫的分子机制提供新线索。
