Institute of Microbiology, Jiangxi Academy of Sciences, No. 7777 Changdong Road, Nanchang, 330096, China.
School of Life Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China.
Virol J. 2021 Dec 14;18(1):250. doi: 10.1186/s12985-021-01721-x.
Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported.
To better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq.
The results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively.
These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.
夹竹桃天蛾 Cypovirus-23（DnCPV-23）是一种新型 Cypovirus，对以夹竹桃和长春花等植物叶片为食的夹竹桃天蛾具有致死作用。在 DnCPV-23 感染后，尚未有关于 Daphnis nerii 反应变化的报道。
为了更好地了解 DnCPV-23 感染的致病机制，本研究采用 3 龄 Daphnis nerii 幼虫经口感染 DnCPV-23 包埋体，72 h 后利用 RNA-seq 分析感染后 Daphnis nerii 中肠的转录组响应。
结果显示，在感染的中肠中共鉴定出 1979 个差异表达的 Daphnis nerii 转录本。KEGG 分析表明，蛋白消化和吸收、Toll 和 Imd 信号通路均下调。基于这一结果，我们推测病毒感染后昆虫中肠的食物消化和吸收可能受到损害。此外，免疫反应的下调可能使 D. nerii 更容易受到细菌感染。甘油磷脂代谢和外源化学物质代谢上调。这两种类型的途径可能分别影响昆虫的病毒复制和外源化学物质解毒。
这些结果可能有助于更好地理解 Cypovirus 感染期间 Daphnis nerii 代谢的变化，并为未来研究 DnCPV-23 入侵的分子机制提供基础。
