使用活性氧激活荧光探针进行视网膜氧化应激的体内成像

In Vivo Imaging of Retinal Oxidative Stress Using a Reactive Oxygen Species-Activated Fluorescent Probe.

作者信息

Prunty Megan C, Aung Moe H, Hanif Adam M, Allen Rachael S, Chrenek Micah A, Boatright Jeffrey H, Thule Peter M, Kundu Kousik, Murthy Niren, Pardue Machelle T

机构信息

Department of Ophthalmology, Emory University, Atlanta, Georgia, United States.

Atlanta VA Center for Visual and Neurocognitive Rehabilitation, Atlanta, Georgia, United States.

出版信息

Invest Ophthalmol Vis Sci. 2015 Sep;56(10):5862-70. doi: 10.1167/iovs.15-16810.

DOI:10.1167/iovs.15-16810

PMID:26348635

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4566416/

Abstract

PURPOSE

In vivo methods for detecting oxidative stress in the eye would improve screening and monitoring of the leading causes of blindness: diabetic retinopathy, glaucoma, and age-related macular degeneration.

METHODS

To develop an in vivo biomarker for oxidative stress in the eye, we tested the efficacy of a reactive oxygen species (ROS)-activated, near-infrared hydrocyanine-800CW (H-800CW) fluorescent probe in light-induced retinal degeneration (LIRD) mouse models. After intravitreal delivery in LIRD rats, fluorescent microscopy was used to confirm that the oxidized H-800CW appeared in the same retinal layers as an established ROS marker (dichlorofluorescein).

RESULTS

Dose-response curves of increasing concentrations of intravenously injected H-800CW demonstrated linear increases in both intensity and total area of fundus hyperfluorescence in LIRD mice, as detected by scanning laser ophthalmoscopy. Fundus hyperfluorescence also correlated with the duration of light damage and functional deficits in vision after LIRD. In LIRD rats with intravitreal injections of H-800CW, fluorescent labeling was localized to photoreceptor inner segments, similar to dichlorofluorescein.

CONCLUSIONS

Hydrocyanine-800CW detects retinal ROS in vivo and shows potential as a novel biomarker for ROS levels in ophthalmic diseases.

摘要

目的

用于检测眼部氧化应激的体内方法将改善对致盲主要原因（糖尿病性视网膜病变、青光眼和年龄相关性黄斑变性）的筛查和监测。

方法

为了开发一种用于眼部氧化应激的体内生物标志物，我们在光诱导视网膜变性（LIRD）小鼠模型中测试了一种活性氧（ROS）激活的近红外花菁-800CW（H-800CW）荧光探针的功效。在向LIRD大鼠玻璃体内注射后，使用荧光显微镜确认氧化的H-800CW出现在与已确立的ROS标志物（二氯荧光素）相同的视网膜层中。