Han Jae Yong, Lee Hyo Gun, Park Young Hyun, Hwang Young Sun, Kim Sang Kyung, Rengaraj Deivendran, Cho Byung Wook, Lim Jeong Mook
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826 Korea.
2Institute for Biomedical Sciences, Shinshu University, Minamiminowa, Nagano, 399-4598 Japan.
J Anim Sci Biotechnol. 2018 Apr 10;9:31. doi: 10.1186/s40104-018-0246-0. eCollection 2018.
Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood.
We found that chicken () was expressed in the stages between the pre-ovulatory follicle and oocyte and was continuously detected in Eyal-Giladi and Kochav stage I (EGK.I) to X. However, was not expressed during folliculogenesis, but began to be detectable between EGK.V and VI. Unexpectedly, could not be detected during folliculogenesis and intrauterine embryonic development. Instead of , was maternally inherited and continuously expressed during chicken intrauterine development. In addition, we found that the pluripotency-related genes such as , , , , , and began to be dramatically upregulated between EGK.VI and VIII.
These results suggest that chickens have a unique pluripotent circuitry since maternally inherited c and may play an important role in the initial acquisition of pluripotency. Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VIII.
转录调节因子获得多能性是早期胚胎发育过程中调节细胞命运和谱系特化所必需的初始发育事件。调节鱼类、两栖动物和哺乳动物多能性网络的进化保守核心转录因子已得到阐明。在多能性获得过程中，也存在物种特异性的母系遗传转录因子及其复杂的转录网络。然而，在鸟类中，早期胚胎发育过程中控制多能性获得的核心转录网络尚不清楚。
我们发现鸡（）在排卵前卵泡和卵母细胞之间的阶段表达，并在Eyal-Giladi和Kochav I期（EGK.I）至X期持续检测到。然而，在卵泡发生过程中不表达，但在EGK.V和VI期之间开始可检测到。出乎意料的是，在卵泡发生和子宫内胚胎发育过程中均未检测到。在鸡的子宫内发育过程中，不是，而是母系遗传并持续表达。此外，我们发现多能性相关基因如、、、、和在EGK.VI和VIII期之间开始显著上调。
这些结果表明，鸡具有独特的多能性调控机制，因为母系遗传的c和可能在多能性的初始获得中起重要作用。此外，鸡胚胎中的多能性获得发生在EGK.VI至VIII期左右。
