Chaudhary Ganga Ram, Singh Prabjot, Kaur Gurpreet, Mehta S K, Kumar Sandeep, Dilbaghi Neeraj
Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University , Chandigarh 160 014, India.
Department of Bio and Nano Technology, Guru Jambheshwar University of Science & Technology , Hisar 125 001, Haryana India.
Inorg Chem. 2015 Sep 21;54(18):9002-12. doi: 10.1021/acs.inorgchem.5b01171. Epub 2015 Sep 11.
A one-pot synthesis of solvophobic bisdodecylaminepalladium(II) chloride (complex 1) was performed. Complex 1 was characterized using X-ray crystallography and other techniques, namely, mass spectrometry, Fourier transform infrared, NMR, elemental analysis, etc. A multifaceted approach was taken to explore the potential applications of complex 1. The micellization ability of complex 1 was estimated using conductivity method in n-alcohols. The metallomicelles are formed in alcohols, and the process is thermodynamically spontaneous in nature. Using complex 1 as precursor, palladium (Pd) nanoparticles were fabricated using two-phase redox method, where reduction is being performed in core of metallomicelles formed by complex 1 in dichloromethane (DCM). The micellization in DCM is confirmed by small-angle X-ray scattering (SAXS). The SAXS measurements reveal that the micellar of core 4-5 nm is being formed, which further controls the size of nanoparticle. This approach was advantageous in terms of size control, methodology, and yield. Pd nanoparticles were characterized using transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and UV-visible spectroscopy and were also screened for bovine serum albumin interactions. Complex 1 and Pd nanoparticles were found to possess antimicrobial property with broad spectrum and are active against bacteria and fungi. The cytotoxicity analyses were performed over healthy cells (Vero cell lines extracted from kidney of green monkey), and the results reveal IC50 value of 10 μg/mL for complex 1.
进行了疏溶剂型双十二烷基胺氯化钯（II）（配合物1）的一锅法合成。使用X射线晶体学和其他技术对配合物1进行了表征，这些技术包括质谱、傅里叶变换红外光谱、核磁共振、元素分析等。采用多方面的方法来探索配合物1的潜在应用。使用电导率法在正醇中估计配合物1的胶束化能力。在醇中形成金属胶束，并且该过程在本质上是热力学自发的。以配合物1为前体，采用两相氧化还原法制备钯（Pd）纳米颗粒，其中还原反应在二氯甲烷（DCM）中由配合物1形成的金属胶束的核心中进行。通过小角X射线散射（SAXS）证实了在DCM中的胶束化。SAXS测量表明形成了核心为4 - 5 nm的胶束，这进一步控制了纳米颗粒的尺寸。这种方法在尺寸控制、方法和产率方面具有优势。使用透射电子显微镜、能量色散X射线光谱、X射线衍射和紫外可见光谱对Pd纳米颗粒进行了表征，并且还筛选了它们与牛血清白蛋白的相互作用。发现配合物1和Pd纳米颗粒具有广谱抗菌性能，对细菌和真菌都有活性。对健康细胞（从绿猴肾脏提取的Vero细胞系）进行了细胞毒性分析，结果显示配合物1的IC50值为10 μg/mL。
