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温室实验环境参数测量与报告指南。

Guidelines for measuring and reporting environmental parameters for experiments in greenhouses.

作者信息

Both A J, Benjamin L, Franklin J, Holroyd G, Incoll L D, Lefsrud M G, Pitkin G

机构信息

NCERA-101, Department of Environmental Sciences, Rutgers University, New Brunswick, NJ USA.

Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ UK.

出版信息

Plant Methods. 2015 Sep 11;11:43. doi: 10.1186/s13007-015-0083-5. eCollection 2015.

Abstract

BACKGROUND

The importance of appropriate, accurate measurement and reporting of environmental parameters in plant sciences is a significant aspect of quality assurance for all researchers and their research. There is a clear need for ensuring research across the world can be compared, understood and where necessary replicated by fellow researchers. A common set of guidelines to educate, assist and encourage comparativeness is of great importance. On the other hand, the level of effort and attention to detail by an individual researcher should be commensurate with the particular research being conducted. For example, a researcher focusing on interactions of light and temperature should measure all relevant parameters and report a measurement summary that includes sufficient detail allowing for replication. Such detail may be less relevant when the impact of environmental parameters on plant growth and development is not the main research focus. However, it should be noted that the environmental experience of a plant during production can have significant impact when subsequent experiments investigate plants at a molecular, biochemical or genetic level or where species interactions are considered. Thus, researchers are encouraged to make a critical assessment of what parameters are of primary importance in their research and these parameters should be measured and reported.

CONTENT

This paper brings together a collection of parameters that the authors, as members of International Committee on Controlled Environment Guidelines (ICCEG) in consultation with members of our three parent organizations, believe constitute those which should be recorded and reported when publishing scientific data from experiments in greenhouses. It provides recommendations to end users on when, how and where these parameters should be measured along with the appropriate internationally standardized units that should be used.

摘要

背景

在植物科学中,对环境参数进行恰当、准确的测量和报告,对于所有研究人员及其研究工作的质量保证而言是一个重要方面。显然有必要确保世界各地的研究能够被同行研究人员进行比较、理解,并在必要时进行重复。一套通用的指导方针对于开展教育、提供协助并鼓励可比性至关重要。另一方面,单个研究人员付出的努力程度和对细节的关注应与所进行的特定研究相匹配。例如,专注于光与温度相互作用的研究人员应测量所有相关参数,并报告包含足够细节以便重复的测量总结。当环境参数对植物生长和发育的影响并非主要研究重点时,此类细节可能就不那么重要了。然而,应当注意的是,当后续实验在分子、生化或遗传水平上研究植物或考虑物种相互作用时,植物在生产过程中的环境经历可能会产生重大影响。因此,鼓励研究人员对其研究中哪些参数至关重要进行批判性评估,并对这些参数进行测量和报告。

内容

本文汇集了一系列参数,作为国际可控环境指南委员会(ICCEG)的成员,作者与我们三个母体组织的成员协商后认为,这些参数构成了在发表温室实验科学数据时应记录和报告的参数。它就何时、如何以及在何处测量这些参数,以及应使用的适当国际标准单位,向最终用户提供了建议。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/4567830/4ed4d358141a/13007_2015_83_Fig1_HTML.jpg

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本文引用的文献

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The art of growing plants for experimental purposes: a practical guide for the plant biologist.
Funct Plant Biol. 2012 Nov;39(11):821-838. doi: 10.1071/FP12028.

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