Røken Mari, Iakhno Stanislav, Haaland Anita Haug, Wasteson Yngvild, Bjelland Ane Mohn
Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 1433 Ås, Norway.
Research and Development, Previwo AS, 0454 Oslo, Norway.
Antibiotics (Basel). 2022 May 10;11(5):637. doi: 10.3390/antibiotics11050637.
Dogs with methicillin-resistant spp. (MRS) infections often undergo treatment in their homes, interacting with their owners and surroundings. This close contact between dogs and owners may facilitate the interspecies transmission of MRS. Therefore, this study aimed to investigate the transmission of MRS from infected dogs to their owners and home environments. Seven households with dogs that had been diagnosed with methicillin-resistant (MRSP) and one household with a dog with methicillin-resistant (MRSE) participated in the study. Dogs, owners, and the home environments were screened for the presence of clinical MRS. A selection of 36 staphylococcal isolates were whole-genome sequenced and screened for resistance genes and virulence genes. Clinical MRS were primarily identified from the dogs and their immediate surroundings, but these were also detected in locations that were out of reach for the dogs, indicating indirect transmission. Two of eight owners carried clinical MRS in their nostrils, while one owner carried methicillin-susceptible (MSSP). All clinical MRS were multi-resistant, and several possessed resistance genes that were not expressed phenotypically. Clinical MRSP persisted in the home environment for a prolonged period, despite infection recovery and one dog being euthanized. Regardless of the stable presence of MRSP in the surroundings, the owners in these homes remained negative, but tested positive for MSSP on three occasions.
感染耐甲氧西林葡萄球菌属（MRS）的犬只通常在家中接受治疗，与主人及周围环境有互动。犬只与主人之间的这种密切接触可能会促进MRS在种间传播。因此，本研究旨在调查MRS从感染犬只传播至其主人及家庭环境的情况。七户饲养已被诊断为耐甲氧西林葡萄球菌（MRSP）犬只的家庭以及一户饲养耐甲氧西林表皮葡萄球菌（MRSE）犬只的家庭参与了该研究。对犬只、主人和家庭环境进行临床MRS筛查。选取36株葡萄球菌分离株进行全基因组测序，并筛查耐药基因和毒力基因。临床MRS主要在犬只及其直接周围环境中被鉴定出来，但在犬只够不到的地方也检测到了这些病菌，这表明存在间接传播。八位主人中有两位在鼻孔中携带临床MRS，而一位主人携带甲氧西林敏感葡萄球菌（MSSP）。所有临床MRS均具有多重耐药性，并且有几种携带未表现出表型的耐药基因。尽管感染已恢复且有一只犬只被安乐死，但临床MRSP仍在家庭环境中持续存在很长时间。尽管周围环境中MRSP稳定存在，但这些家庭中的主人检测结果仍为阴性，但有三次检测出MSSP呈阳性。
