Department of Laboratory Medicine, Taipei Medical University Hospital, Taipei, Taiwan.
Int J Nanomedicine. 2012;7:4335-40. doi: 10.2147/IJN.S31030. Epub 2012 Aug 6.
Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum.
The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference.
In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay.
We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.
经过抗体生物功能化的磁性纳米粒子能够识别并结合相应的抗原。在这项工作中,将抗 C 反应蛋白(CRP)抗体共价偶联到磁性纳米粒子表面,以特异性标记血清中的 CRP。
通过免疫磁还原(IMR)检测来检测血清 CRP 水平,该方法可识别代表抗体偶联磁性纳米粒子与 CRP 蛋白之间相互作用水平的磁信号变化。为了研究 IMR 在临床应用中的可行性,我们用 IMR 检测了纯 CRP 溶液和 40 个人血清样本中的 CRP,以评估其灵敏度、特异性和干扰情况。
与免疫比浊法相比,IMR 检测法的结果显示出更高的灵敏度,并且与当前免疫比浊法的检测结果高度相关。
我们已经开发出一种新颖且有前途的方法,可通过临床诊断中的免疫磁还原法检测人血清中的 CRP。