Sun W J, Sha Z F, Gao H
Shaanxi Provincial Institute for Drug Control, Xian.
Yao Xue Xue Bao. 1990;25(7):530-3.
The main active constituents--osthol and imperatorin were isolated from Cnidium monnieri (L.) Cuss and then used as standards for their determination in the crude drug by fluorometry TLCS. The fructus Cnidii was macerated with ethanol overnight and the ethanolic extract was spotted on a silica gel G-CMC Na plate. The plate was developed with n-hexane-ethyl acetate (7:3). The spot was excited by light of 365 nm, and determined in a Shimadzu CS-930 TLC scanner using a No. 2 filter. The peak areas of the two components were obtained, external standard method was used in the determination. The root, stem, leaf and flower of this plant were shown to contain no osthol and imperatorin, and the seed was found to contain 2.0-3.2% of osthol and 1.8-2.5% of imperatorin.
从蛇床子中分离出主要活性成分蛇床子素和欧前胡素，然后用作荧光薄层扫描法测定该药材中这两种成分的对照品。蛇床子果实用乙醇浸泡过夜，乙醇提取物点于硅胶G-CMC Na板上。用正己烷-乙酸乙酯（7:3）展开。斑点用365nm的光激发，在岛津CS-930薄层扫描仪上用2号滤光片测定。得到两种成分的峰面积，测定采用外标法。结果表明，该植物的根、茎、叶和花中不含蛇床子素和欧前胡素，种子中蛇床子素含量为2.0-3.2%，欧前胡素含量为1.8-2.5%。
