Tahmassebi J F, Drogkari E, Wood S R
Department of Paediatric Dentistry, Leeds Dental Institute, Clarendon Way, Leeds, LS2 9LU, UK.
Division of Oral Biology, Leeds Dental Institute, University of Leeds, Clarendon Way, Leeds, LS2 9LU, UK.
Eur Arch Paediatr Dent. 2015 Dec;16(6):433-40. doi: 10.1007/s40368-014-0165-5. Epub 2015 Sep 18.
The aim of this study was to provide preliminary data on the most effective erythrosine concentration and light dose for the erythrosine-based photodynamic therapy (PDT) of oral plaque biofilms formed in vivo.
A randomised controlled study with 15 volunteers was carried out to investigate the effect of photo-sensitiser and light dose on the killing of bacteria in oral plaque biofilms formed in vivo. All volunteers wore a removable in situ appliance carrying six enamel slabs for two phases of 2 weeks each. During this time, plaque biofilms accumulated on the enamel slabs. The slabs were then removed from the appliances for PDT treatment in vitro. In the first phase of the study, erythrosine doses of 22 and 220 μM were used for the photodynamic treatment of the biofilms. In the second phase, the erythrosine concentration was kept constant, and the light dose was varied. Following treatment, the biofilms were disaggregated, and the total bacterial killing was determined using colony counting.
The erythrosine dose of 220 μM caused the most cell killing relative to controls. Fifteen minutes of continuous irradiation with light and light fractionation of 5 × 1 min irradiation separated by 2-min-dark recovery periods were found to be the most effective bactericidal regimes.
Erythrosine-based PDT shows promise as an antibacterial treatment for oral plaque biofilms. Further research is needed to prove its clinical and cost-effectiveness compared with current best practice.
本研究旨在提供关于基于赤藓红的光动力疗法（PDT）治疗体内形成的口腔菌斑生物膜的最有效赤藓红浓度和光剂量的初步数据。
对15名志愿者进行了一项随机对照研究，以调查光敏剂和光剂量对体内形成的口腔菌斑生物膜中细菌杀灭的影响。所有志愿者佩戴一个可摘戴的原位矫治器，该矫治器带有六块牙釉质板，分两个阶段，每个阶段为期2周。在此期间，牙菌斑生物膜在牙釉质板上积聚。然后将这些板从矫治器上取下，在体外进行PDT治疗。在研究的第一阶段，使用22和220μM的赤藓红剂量对生物膜进行光动力治疗。在第二阶段，赤藓红浓度保持不变，光剂量则有所变化。治疗后，将生物膜分解，并通过菌落计数确定细菌的总杀灭情况。
相对于对照组，220μM的赤藓红剂量导致的细胞杀灭最多。发现连续照射15分钟以及5×1分钟照射且每次照射后有2分钟黑暗恢复期的分次照射是最有效的杀菌方案。
基于赤藓红的PDT作为口腔菌斑生物膜的抗菌治疗方法显示出前景。与当前最佳实践相比，还需要进一步研究以证明其临床效果和成本效益。
