Peng Po-Chun, Hsieh Chien-Ming, Chen Chueh-Pin, Tsai Tsuimin, Chen Chin-Tin
Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Department of Cosmetic Science, Providence University, Taichung City 43301, Taiwan.
Int J Mol Sci. 2016 Nov 1;17(11):1821. doi: 10.3390/ijms17111821.
Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of (), (), and (). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease.
制备了含有羟丙基甲基纤维素（HPMC）和甲苯胺蓝O的壳聚糖水凝胶，并评估了其粘膜粘附性能和光动力灭活（PDI）的抗菌效果。水凝胶中HPMC含量的增加导致粘膜粘附性增强。此外，我们开发了一种类似于口腔牙周袋的简单体外3D牙龈模型，用于培养（）、（）和（）的生物膜。研究了壳聚糖水凝胶对在这种3D牙龈模型中培养的牙周生物膜的PDI效果。我们发现，由于在未照射部位仍有一些最内层细菌存活，PDI效果有限。利用这种3D牙龈模型，我们通过对光能和照射部位的各种调整进一步优化了PDI程序。壳聚糖水凝胶对牙周生物膜的PDI效果可通过四面照射显著提高。总之，本研究不仅展示了这种壳聚糖水凝胶的临床适用性，还展示了光照射模式在牙周疾病PDI治疗中的重要性。
