Fracalossi Camila, Nagata Juliana Yuri, Pellosi Diogo Silva, Terada Raquel Sano Suga, Hioka Noboru, Baesso Mauro Luciano, Sato Francielle, Rosalen Pedro Luiz, Caetano Wilker, Fujimaki Mitsue
Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil.
Endodontic Department, Federal University of Sergipe, Avenida Marechal Rondon, S/n - Jardim Rosa Elze, Largarto, São Cristóvão, SE, Cep 49100-000, Brazil.
Photodiagnosis Photodyn Ther. 2016 Sep;15:127-32. doi: 10.1016/j.pdpdt.2016.06.011. Epub 2016 Jun 30.
Photodynamic inactivation of microorganisms is based on a photosensitizing substance which, in the presence of light and molecular oxygen, produces singlet oxygen, a toxic agent to microorganisms and tumor cells. This study aimed to evaluate singlet oxygen quantum yield of erythrosine solutions illuminated with a halogen light source in comparison to a LED array (control), and the photodynamic effect of erythrosine dye in association with the halogen light source on Streptococcus mutans.
Singlet oxygen quantum yield of erythrosine solutions was quantified using uric acid as a chemical-probe in an aqueous solution. The in vitro effect of the photodynamic antimicrobial activity of erythrosine in association with the halogen photopolimerizing light on Streptococcus mutans (UA 159) was assessed during one minute. Bacterial cultures treated with erythrosine alone served as negative control.
Singlet oxygen with 24% and 2.8% degradation of uric acid in one minute and a quantum yield of 0.59 and 0.63 was obtained for the erythrosine samples illuminated with the halogen light and the LED array, respectively. The bacterial cultures with erythrosine illuminated with the halogen light presented a decreased number of CFU mL(-1) in comparison with the negative control, with minimal inhibitory concentrations between 0.312 and 0.156mgmL(-1).
The photodynamic response of erythrosine induced by the halogen light was capable of killing S. mutans. Clinical trials should be conducted to better ascertain the use of erythrosine in association with halogen light source for the treatment of dental caries.
微生物的光动力失活基于一种光敏物质，该物质在光和分子氧存在的情况下会产生单线态氧，这是一种对微生物和肿瘤细胞有毒的物质。本研究旨在评估与发光二极管阵列（对照）相比，用卤素光源照射的赤藓红溶液的单线态氧量子产率，以及赤藓红染料与卤素光源联合对变形链球菌的光动力效应。
在水溶液中使用尿酸作为化学探针来定量赤藓红溶液的单线态氧量子产率。评估了赤藓红与卤素光聚合光联合对变形链球菌（UA 159）的光动力抗菌活性的体外效应，持续时间为一分钟。仅用赤藓红处理的细菌培养物作为阴性对照。
用卤素光和发光二极管阵列照射的赤藓红样品，在一分钟内尿酸降解率分别为24%和2.8%，量子产率分别为0.59和0.63。与阴性对照相比，用卤素光照射的含赤藓红的细菌培养物的CFU mL(-1)数量减少，最低抑菌浓度在0.312至0.156mgmL(-1)之间。
卤素光诱导的赤藓红的光动力反应能够杀死变形链球菌。应进行临床试验，以更好地确定赤藓红与卤素光源联合用于治疗龋齿的用途。
