Gong Jungeun, Park Howon, Lee Juhyun, Seo Hyunwoo, Lee Siyoung
1 Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea.
2 Department of Microbiology and Immunology, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea.
Photobiomodul Photomed Laser Surg. 2019 May;37(5):282-287. doi: 10.1089/photob.2018.4571. Epub 2019 Apr 5.
The aim of this study was to evaluate the effect of photodynamic therapy (PDT) on multispecies oral caries biofilms composed of , , and . The abovementioned microorganisms largely cause dental caries, especially early childhood caries (ECC), by synthesizing of acids in the presence of sugar. PDT is considered an effective process to remove oral biofilms, and erythrosine, an oral bacterial disclosing agent, is an ideal dye that can be used as a photosensitizer in PDT. However, until now, there are no studies that have reported the effect of erythrosine-mediated PDT on biofilms, including the three microorganisms. The biofilms were formed on hydroxyapatite discs, and erythrosine was used as the photosensitizer, diluted to a concentration of 40 μM for 3 min. Light was irradiated for 10 and 20 sec using a blue light-emitting diode dental curing light. After the experiment, the colony-forming units of each microbial group cultured on blood agar plates were counted, and a confocal laser-scanning microscope was used to evaluate the effect of PDT. The counts of all three microorganisms significantly decreased in the PDT group compared with those in the control group. For and , there was a larger decrease proportional to the amount of energy irradiated. Overall, PDT showed a significant antimicrobial effect against oral biofilms composed of the three microorganisms, suggesting its potential clinical application for infants with ECC.
本研究的目的是评估光动力疗法（PDT）对由[具体菌种1]、[具体菌种2]和[具体菌种3]组成的多菌种口腔龋生物膜的影响。上述微生物在有糖存在的情况下通过合成酸在很大程度上导致龋齿，尤其是幼儿龋齿（ECC）。PDT被认为是去除口腔生物膜的有效方法，而赤藓红作为一种口腔细菌显色剂，是一种理想的染料，可在PDT中用作光敏剂。然而，到目前为止，尚无研究报道赤藓红介导的PDT对包括这三种微生物在内的生物膜的影响。生物膜在羟基磷灰石圆盘上形成，使用赤藓红作为光敏剂，稀释至浓度为40μM，作用3分钟。使用蓝光发光二极管牙科固化灯照射10秒和20秒。实验结束后，对在血琼脂平板上培养的每个微生物组的菌落形成单位进行计数，并使用共聚焦激光扫描显微镜评估PDT的效果。与对照组相比，PDT组中所有三种微生物的计数均显著降低。对于[具体菌种1]和[具体菌种2]，与照射的能量量成比例地有更大的减少。总体而言，PDT对由这三种微生物组成的口腔生物膜显示出显著的抗菌作用，表明其在患有ECC的婴儿中的潜在临床应用。
