失调的miR-645影响头颈癌细胞的增殖和侵袭。
Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell.
作者信息
Sun Qiang, Chen Shuai, Zhao Xue, Yan Ming, Fang Zheng, Wang Haibin, Zhao Junfang, Sun Minglei, Han Xinguang, Chen Wantao, Li Xinming
机构信息
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
出版信息
Cancer Cell Int. 2015 Sep 17;15:87. doi: 10.1186/s12935-015-0238-5. eCollection 2015.
BACKGROUND AND PURPOSE
Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function.
METHODS
The association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan-Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells.
RESULTS
In the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration.
CONCLUSIONS
miR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC.
背景与目的
失调的微小RNA（miRNA）在许多恶性肿瘤中发挥重要作用。然而，阐明miRNA在癌症生物学中的作用，尤其是在上皮性癌症中的作用，仍是一个持续的过程。在本研究中，我们鉴定了在头颈部鳞状细胞癌（HNSCC）进展过程中差异表达的miR-645，并研究了其生物学功能。
方法
采用Kaplan-Meier生存分析，分析临床病理参数与候选miRNA表达水平之间的关联。观察细胞生长、侵袭和迁移潜能以及克隆形成，以检测miRNA在HNSCC细胞中的功能。
结果
在34例有淋巴结转移的HNSCC组织中，miR-645的表达水平为0.54±0.12，在28例无淋巴结转移的组织中，表达水平为0.22±0.05（p = 0.017）。在HNSCC患者中，miR-645表达水平较高与总体生存率较差显著相关（p = 0.04）。miR-645的异位表达促进了细胞侵袭和迁移。
结论
miR-645在细胞侵袭和转移中起关键作用，其表达与HNSCC患者的总体生存率相关。
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