采用高分辨率液相色谱-轨道阱质谱法检测玉米中的N-(1-脱氧-D-果糖-1-基)伏马毒素B₂和B₃
Detection of N-(1-deoxy-D-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS.
作者信息
Matsuo Yosuke, Takahara Kentaro, Sago Yuki, Kushiro Masayo, Nagashima Hitoshi, Nakagawa Hiroyuki
机构信息
National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan.
Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan.
出版信息
Toxins (Basel). 2015 Sep 16;7(9):3700-14. doi: 10.3390/toxins7093700.
The existence of glucose conjugates of fumonisin B₂ (FB₂) and fumonisin B₃ (FB₃) in corn powder was confirmed for the first time. These "bound-fumonisins" (FB₂ and FB₃ bound to glucose) were identified as N-(1-deoxy-D-fructos-1-yl) fumonisin B₂ (NDfrc-FB₂) and N-(1-deoxy-D-fructos-1-yl) fumonisin B₃ (NDfrc-FB₃) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB₂ and NDfrc-FB₃ with the o-phthalaldehyde (OPA) reagent also supported that D-glucose binding to FB₂ and FB₃ molecules occurred to their primary amine residues.
首次证实了玉米粉中存在伏马菌素B₂（FB₂）和伏马菌素B₃（FB₃）的葡萄糖共轭物。这些“结合型伏马菌素”（FB₂和FB₃与葡萄糖结合）分别被鉴定为N-（1-脱氧-D-果糖-1-基）伏马菌素B₂（NDfrc-FB₂）和N-（1-脱氧-D-果糖-1-基）伏马菌素B₃（NDfrc-FB₃），这是基于使用高分辨率液相色谱-轨道阱质谱（LC-Orbitrap MS）分析对特征离子的精确质量测量和碎片模式得出的。用邻苯二甲醛（OPA）试剂处理NDfrc-FB₂和NDfrc-FB₃也支持D-葡萄糖与FB₂和FB₃分子的伯胺残基发生了结合。
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