Yarnpakdee Suthasinee, Benjakul Soottawat, Kristinsson Hordur G, Bakken Hilma Eiðsdóttir
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand.
Division of Biotechnology and Biomolecules, Matis - Icelandic Food and Biotechnology R & D, Vinlandsleid 12, 113 Reykjavik, Iceland ; Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611 USA.
J Food Sci Technol. 2015 Oct;52(10):6194-205. doi: 10.1007/s13197-014-1672-4. Epub 2015 Jan 15.
Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H2O2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P > 0.05). Cell reactive oxygen species (ROS) generation as mediated by H2O2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H2O2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.
采用化学模型和细胞模型研究了用碱性蛋白酶（HA）、碱性蛋白酶后接木瓜蛋白酶（HAPa）从尼罗罗非鱼分离蛋白制备的蛋白水解物及其葡聚糖凝胶G - 25级分（FHA和FHAPa）的抗氧化活性。在所有样品中，FHAPa表现出最高的化学抗氧化活性，但它没有金属螯合活性。测试了HA、HAPa及其级分对H2O2和AAPH诱导的HepG2细胞氧化损伤和DNA损伤的细胞抗氧化能力。当在不存在和存在50 μM Trolox的情况下用不同浓度（0.5 - 2 mg/mL）的所有水解物或级分预处理细胞时，细胞活力在91.10 - 111.40%范围内。然而，不同浓度的样品之间未观察到细胞活力的差异（P＞0.05）。用水解物或其级分处理后，尤其是与50 μM Trolox联合处理时，由H2O2和AAPH介导的细胞活性氧（ROS）生成减少。FHAPa以剂量依赖的方式有效抑制H2O2和过氧自由基诱导的DNA断裂。因此，尼罗罗非鱼蛋白水解物可作为一种功能性食品成分。
