Marine Biochemistry Laboratory, Department of Chemistry, Pukyong National University , Busan 608-737, South Korea.
J Agric Food Chem. 2012 Sep 12;60(36):9112-9. doi: 10.1021/jf302161m. Epub 2012 Aug 27.
Gelatin was extracted from the skin of Japanese flounder ( Palatichtys olivaceus ) and was subjected to enzymatic hydrolysis. The peptic hydrolysate resulted in a potent antioxidative peptide Gly-Gly-Phe-Asp-Met-Gly (582 Da), which bears +12.61 kcal/mol hydrophobicity. The antioxidative potential of the peptide was characterized by analyzing the protective effect of the peptide on reactive oxygen species (ROS)-mediated intracellular macromolecule damage. It was found that the peptide is a potent scavenger of intracellular ROS, thereby protecting the radical-mediated damage of membrane lipids, proteins, and DNA. Moreover, the peptide is capable of upregulating the expression of inherent antioxidative enzymes, superoxide dismutase-1, glutathione, and catalase. Collectively, it can be concluded that Japanese flounder skin, a processing byproduct of filleting, can be effectively used to produce a bioactive peptide with potent antioxidant capacity.
明胶是从日本牙鲆（Palatichtys olivaceus）的皮中提取的，并进行了酶解。胃蛋白酶水解产物得到了一种具有强大抗氧化活性的肽 Gly-Gly-Phe-Asp-Met-Gly（582 Da），其疏水性为+12.61 kcal/mol。通过分析该肽对活性氧（ROS）介导的细胞内大分子损伤的保护作用，研究了该肽的抗氧化潜力。结果发现，该肽是细胞内 ROS 的有效清除剂，从而保护了膜脂质、蛋白质和 DNA 的自由基介导的损伤。此外，该肽还能上调固有抗氧化酶超氧化物歧化酶-1、谷胱甘肽和过氧化氢酶的表达。综上所述，可以得出结论，鱼片加工的副产品日本牙鲆皮可以有效地用来生产具有强大抗氧化能力的生物活性肽。
