Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.
Oxid Med Cell Longev. 2021 Aug 22;2021:9987844. doi: 10.1155/2021/9987844. eCollection 2021.
In this work, the antioxidant mechanisms of bioactive oligopeptides (FWKVV and FMPLH) from protein hydrolysate of miiuy croaker muscle against HO-damaged human umbilical vein endothelial cells (HUVECs) were researched systemically. The finding demonstrated that the HUVEC viability treated with ten antioxidant peptides (M1 to M10) at 100.0 M for 24 h was not significantly affected compared with that of the normal group ( < 0.05). Furthermore, FWKVV and FMPLH at 100.0 M could very significantly enhance the viabilities (75.89 ± 1.79% and 70.03 ± 4.37%) of oxidative-damaged HUVECs by HO compared with those of the model group (51.66 ± 2.48%) ( < 0.001). The results indicated that FWKVV and FMPLH played their protective functions through increasing the levels of antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreasing the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and nitric oxide (NO) in oxidative-damaged HUVECs in a dose-dependent manner. In addition, the comet assay revealed that FWKVV and FMPLH could dose-dependently protect deoxyribonucleic acid (DNA) from oxidative damage in the HUVEC model. These results suggested that antioxidant pentapeptides (FWKVV and FMPLH) could serve as potential antioxidant additives applied in the food products, pharmaceuticals, and health supplements.
在这项工作中，系统研究了来自米鱼肌肉蛋白水解物的生物活性寡肽 (FWKVV 和 FMPLH) 对 HO 损伤的人脐静脉内皮细胞 (HUVEC) 的抗氧化机制。研究结果表明，在 100.0μM 下用 10 种抗氧化肽 (M1 至 M10) 处理 24 小时后，HUVEC 活力与正常组相比没有显著变化（<0.05）。此外，与模型组（51.66±2.48%）相比，100.0μM 的 FWKVV 和 FMPLH 可非常显著地提高 HO 致氧化损伤的 HUVEC 活力（75.89±1.79%和 70.03±4.37%）（<0.001）。结果表明，FWKVV 和 FMPLH 通过增加抗氧化酶（包括超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GSH-Px)）的水平，降低活性氧 (ROS)、丙二醛 (MDA) 和一氧化氮 (NO) 的水平，发挥其保护功能在氧化损伤的 HUVEC 中呈剂量依赖性。此外，彗星试验表明，FWKVV 和 FMPLH 可以剂量依赖性地保护 HUVEC 模型中的脱氧核糖核酸 (DNA) 免受氧化损伤。这些结果表明，抗氧化五肽 (FWKVV 和 FMPLH) 可以作为潜在的抗氧化添加剂应用于食品、药品和保健品中。
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