College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.
Nutrients. 2019 Feb 13;11(2):392. doi: 10.3390/nu11020392.
According to a previous study, YGDEY from tilapia fish skin gelatin hydrolysates has strong free radical scavenging activity. In the present study, the protective effect of YGDEY against oxidative stress induced by ethanol in HepG2 cells was investigated. First, cells were incubated with YGDEY (10, 20, 50, and 100 μM) to assess cytotoxicity, and there was no significant change in cell viability. Next, it was established that YGDEY decreased the production of reactive oxygen species (ROS). Western blot results indicated that YGDEY increased the levels of superoxide dismutase (SOD) and glutathione (GSH) and decreased the expression of gamma-glutamyltransferase (GGT) in HepG2 cells. It was then revealed that YGDEY markedly reduced the expressions of bax and cleaved-caspase-3 (c-caspase-3); inhibited phosphorylation of Akt, IκB-α, p65, and p38; and increased the level of bcl-2. Moreover, the comet assay showed that YGDEY effectively decreased the amount of ethanol-induced DNA damage. Thus, YGDEY protected HepG2 cells from alcohol-induced injury by inhibiting oxidative stress, and this may be associated with the Akt/nuclear factor-κB (NF-κB)/mitogen-activated protein kinase (MAPK) signal transduction pathways. These results demonstrate that YGDEY from tilapia fish skin gelatin hydrolysates protects HepG2 cells from oxidative stress, making it a potential functional food ingredient.
根据先前的一项研究，来自罗非鱼鱼皮明胶水解物的 YGDEY 具有很强的自由基清除活性。本研究探讨了 YGDEY 对 HepG2 细胞乙醇诱导氧化应激的保护作用。首先，用 YGDEY（10、20、50 和 100 μM）孵育细胞以评估细胞毒性，细胞活力没有明显变化。接下来，发现 YGDEY 减少了活性氧（ROS）的产生。Western blot 结果表明，YGDEY 增加了超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平，并降低了 HepG2 细胞中γ-谷氨酰转移酶（GGT）的表达。然后表明，YGDEY 显著降低了 bax 和裂解型 caspase-3（c-caspase-3）的表达；抑制 Akt、IκB-α、p65 和 p38 的磷酸化；并增加了 bcl-2 的水平。此外，彗星试验表明，YGDEY 有效减少了乙醇诱导的 DNA 损伤量。因此，YGDEY 通过抑制氧化应激来保护 HepG2 细胞免受酒精损伤，这可能与 Akt/核因子-κB（NF-κB)/丝裂原活化蛋白激酶（MAPK）信号转导途径有关。这些结果表明，来自罗非鱼鱼皮明胶水解物的 YGDEY 可保护 HepG2 细胞免受氧化应激，使其成为一种有潜力的功能性食品成分。
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