人巨细胞病毒pUL93是病毒基因组切割和包装所必需的。
Human Cytomegalovirus pUL93 Is Required for Viral Genome Cleavage and Packaging.
作者信息
DeRussy Bernadette M, Tandon Ritesh
机构信息
Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA
出版信息
J Virol. 2015 Dec;89(23):12221-5. doi: 10.1128/JVI.02382-15. Epub 2015 Sep 23.
Abstract
Human cytomegalovirus (HCMV) pUL93 is essential for virus growth, but its precise function in the virus life cycle is unknown. Here, we characterize a UL93 stop mutant virus (UL93st-TB40/E-BAC) to demonstrate that the absence of this protein does not restrict viral gene expression; however, cleavage of viral DNA into unit-length genomes as well as genome packaging is abolished. Thus, pUL93 is required for viral genome cleavage and packaging.
摘要
人巨细胞病毒（HCMV）的pUL93对病毒生长至关重要，但其在病毒生命周期中的精确功能尚不清楚。在此，我们对一个UL93缺失突变病毒（UL93st-TB40/E-BAC）进行了特性分析，以证明该蛋白的缺失并不限制病毒基因表达；然而，病毒DNA切割成单位长度基因组以及基因组包装过程被废除。因此，pUL93是病毒基因组切割和包装所必需的。
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