Caneschi Carolina Milagres, Muniyappa Mohan K, Duarte Lucienir P, Silva Grácia D F, Dos Santos Orlando David Henrique, Spillane Charles, Filho Sidney Augusto Vieira
Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil ; Genetics and Biotechnology Lab, Centre for Chromosome Biology, National University of Ireland, Galway, Ireland.
Genetics and Biotechnology Lab, Centre for Chromosome Biology, National University of Ireland, Galway, Ireland.
J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):6-11. doi: 10.5455/jice.20141006105747. Epub 2014 Nov 28.
Aiming the continuity of the studies of Austroplenckia populnea, Brazilian species of the Celastraceae family, in the present study, it was investigated the effect of crude extracts obtained with ethanol, ethyl acetate and chloroform and two purified constituents, proanthocyanidin A and 4'-O-methylepigallocatechin, both isolated from its samaras, on cancer cell proliferation assays.
The human cancer cells lines MCF-7 (ductal breast carcinoma), A549 (lung cancer), HS578T (ductal breast carcinoma) and non-cancer HEK293 (embryonic kidney cells) were treated with different concentrations of extracts and constituents and the effect was observed through the acid phosphatase method. The chemical structures of the purified compounds were identified by the respective IR and (1)H and (13)C nuclear magnetic resonance spectral data.
While crude extracts from samaras of the folk medicine A. populnea can trigger cell proliferative effects in human cell lines, the purified compounds (proanthocyanidin A and 4'-O-methyl-epigallocatechin) isolated from the same extracts can have an opposite (anti-proliferative) effect.
Based on the results, it was possible to suggest that extracts from samaras of A. populnea should be further investigated for possible cancer-promoting activities; and the active extracts can also represent a source of compounds that have anti-cancer properties.
为了延续对南蛇藤科巴西物种奥氏南蛇藤（Austroplenckia populnea）的研究，在本研究中，调查了用乙醇、乙酸乙酯和氯仿获得的粗提物以及从其翅果中分离出的两种纯化成分原花青素A和4'-O-甲基表没食子儿茶素对癌细胞增殖试验的影响。
用不同浓度的提取物和成分处理人癌细胞系MCF-7（乳腺导管癌）、A549（肺癌）、HS578T（乳腺导管癌）和非癌性HEK293（胚胎肾细胞），并通过酸性磷酸酶法观察效果。通过各自的红外光谱以及氢核磁共振和碳核磁共振光谱数据鉴定纯化化合物的化学结构。
虽然民间药物奥氏南蛇藤翅果的粗提物可在人细胞系中引发细胞增殖作用，但从相同提取物中分离出的纯化化合物（原花青素A和4'-O-甲基表没食子儿茶素）可产生相反（抗增殖）作用。
基于这些结果，有可能表明应进一步研究奥氏南蛇藤翅果提取物的潜在促癌活性；而活性提取物也可能代表具有抗癌特性的化合物来源。
