Ohkura Naoki, Yokouchi Haruna, Mimura Mariyo, Nakamura Riki, Atsumi Gen-Ichi
Department of Molecular Physiology and Pathology, School of Pharma-Sciences, Teikyo University, Kaga, Itabashi, Tokyo, Japan.
J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):19-23. doi: 10.5455/jice.20141128032845. Epub 2015 Jan 3.
This study aimed to identify new hemostyptics by assessing the coagulation enhancing activity of 114 Chinese herbal extracts in vitro.
Herbs were boiled in water for 30 min, filtered and then lyophilized filtrates (10 mg/mL) were dissolved in water. Coagulation was assayed as prothrombin time (PT). Plasma diluted in saline was incubated with each extract for 5 min and then PT reagent was added, followed by CaCl2 solution and the time taken to form clots was measured. Extracts that decreased coagulation time were regarded as containing active compounds. The abilities of extracts to activate Factor XII were assessed and the activated form of factor XII (XIIa) was resolved by SDS-PAGE and visualized by silver staining.
Coagulation time was obviously shortened by extracts of Alpinia Rhizome, Areca, Artemisia Leaf, Cassia Bark, Danshen Root, Ephedra Herb, Epimedium Herb, Forsythia Fruit, Great Burdock Achene, Moutan Bark, Perilla Herb, Red Paeony Root, Schizonepeta Spike, Senticosus Rhizome, Sweet Annie, Uncaria Thorn and Zanthoxylum Peel. Factor XII was obviously activated by extracts of Artemisia Leaf and Great Burdock Achene, and slightly by Perilla herb.
Some popular Chinese medicinal herbs have potential as hemostatic agents and could thus be develope as new strategies for the treatment and prevention of bleeding.
本研究旨在通过评估114种中草药提取物的体外促凝血活性来鉴定新的止血剂。
将草药水煮30分钟，过滤，然后将冻干的滤液（10mg/mL）溶于水。以凝血酶原时间（PT）测定凝血情况。将用生理盐水稀释的血浆与每种提取物孵育5分钟，然后加入PT试剂，接着加入氯化钙溶液，并测量形成凝块所需的时间。凝血时间缩短的提取物被视为含有活性化合物。评估提取物激活因子XII的能力，通过SDS-PAGE分离因子XII的活化形式（XIIa），并用银染色法进行可视化。
高良姜、槟榔、艾叶、桂皮、丹参、麻黄、淫羊藿、连翘、牛蒡子、牡丹皮、紫苏、赤芍、荆芥、刺五加、茵陈、钩藤和花椒皮的提取物明显缩短了凝血时间。艾叶和牛蒡子的提取物明显激活了因子XII，紫苏提取物则有轻微激活作用。
一些常用的中草药具有作为止血剂的潜力，因此可开发为治疗和预防出血的新策略。
