Silva Ana Carolina Mazei, Dos Santos Maísa Pavani, de França Suélem Aparecida, da Silva Virginia Claudia, da Silva Luiz Everson, de Figueiredo Uir Santana, Dall'Oglio Evandro Luiz, Júnior Paulo Teixeira de Sousa, Lopes Carbene França, Baviera Amanda Martins, Kawashita Nair Honda
Department of Chemistry, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Department of Science Education, Federal University of Paraná, Coastal Sector, Matinhos, Paraná, Brazil.
J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):57-63. doi: 10.5455/jice.20141028022407. Epub 2014 Nov 28.
The present study was undertaken to evaluate the acute and subchronic antihyperglycemic effects of methanolic extract of Bowdichia virgilioides root bark of B. virgilioides in streptozotocin (STZ)-induced diabetic rats.
The extract (100, 250 or 500 mg/kg) was orally administered to male Wistar diabetic (STZ, 42 mg/kg i.v.) and non-diabetic rats into two main protocols: (i) subchronic experiments, where animals were treated for 21 days with B. virgilioides extract and the following parameters were evaluated: Body weight, fluid and food intake (determined daily), urinary glucose and urea (every 3 days) and glycemia (every 5 days). At the end of the experimental period, skeletal muscles (extensor digitorum longus [EDL] and soleus), retroperitoneal and epididymal white adipose tissues were collected and weighed; liver samples were used for the determination of the lipid and glycogen contents; (ii) acute experiments, which evaluated the alterations on fasting and post-prandial glycemia and on glucose tolerance using the oral glucose tolerance test (OGTT).
In subchronic experiments, the treatment with B. virgilioides extract did not change any parameter evaluated in diabetic and non-diabetic animals. On fasting and post-prandial glycemia, the extract treatment did not promote changes in the glycemia values in diabetic or non-diabetic animals. In OGTT, the treatment with 500 mg/kg B. virgilioides extract reduced the hyperglycemia peak after a glucose overload, when compared with non-treated diabetic animals, resulting in a lower area under curve.
The results of our work indicate that B. virgilioides root extract promotes an acute antihyperglycemic effect in STZ-diabetic rats; this effect probably occurs through an inhibition of the intestinal glucose absorption. The continuity of the research is necessary to elucidate these possibilities.
本研究旨在评估 Virgilioides 豆根皮甲醇提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠的急性和亚慢性降血糖作用。
将提取物（100、250 或 500 mg/kg）口服给予雄性 Wistar 糖尿病（静脉注射 42 mg/kg STZ）和非糖尿病大鼠，分为两个主要方案：（i）亚慢性实验，动物用 Virgilioides 豆提取物治疗 21 天，并评估以下参数：体重、液体和食物摄入量（每日测定）、尿葡萄糖和尿素（每 3 天测定）以及血糖（每 5 天测定）。在实验期结束时，收集并称重骨骼肌（趾长伸肌[EDL]和比目鱼肌）、腹膜后和附睾白色脂肪组织；肝脏样本用于测定脂质和糖原含量；（ii）急性实验，使用口服葡萄糖耐量试验（OGTT）评估空腹和餐后血糖以及葡萄糖耐量的变化。
在亚慢性实验中，用 Virgilioides 豆提取物治疗未改变糖尿病和非糖尿病动物中评估的任何参数。在空腹和餐后血糖方面，提取物治疗未促进糖尿病或非糖尿病动物血糖值的变化。在 OGTT 中，与未治疗的糖尿病动物相比，用 500 mg/kg Virgilioides 豆提取物治疗可降低葡萄糖超载后的高血糖峰值，导致曲线下面积降低。
我们的研究结果表明，Virgilioides 豆根提取物对 STZ 糖尿病大鼠具有急性降血糖作用；这种作用可能通过抑制肠道葡萄糖吸收而发生。有必要继续进行研究以阐明这些可能性。
