Bouadid Ismail, Akdad Mourad, Eddouks Mohamed
Team of Ethnopharmacology and Pharmacognosy, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000, Errachidia, Morocco.
Cardiovasc Hematol Disord Drug Targets. 2022;22(3):168-178. doi: 10.2174/1871529X22666220908104724.
The study aimed to evaluate the glucose-lowering effect of Tetraclinis articulata.
Tetraclinis articulata is commonly used for the treatment of diabetes characterized by chronic hyperglycemia.
This work aimed to evaluate the effect of Tetraclinis articulata (T. articulata) Aqueous Extract (TAAE) on glycemia and lipid profile in normal and Streptozotocin (STZ)-induced diabetic rats. Additionally, its acute toxicity, phytochemical composition, and antioxidant capacity were assessed.
To highlight the effect of TAAE on plasma glucose levels and lipid metabolism, blood glucose levels were measured at 1, 2, 4, and 6 hours of treatment for the acute test and on days 2, 4 and 7 over the daily oral administration for the subchronic test at two selected doses (10 mg/kg and 20 mg/kg). Furthermore, Triglycerides (TGs), Total Cholesterol (TC), and High-Density Lipoprotein cholesterol (HDL-c) were measured after the treatment. The rats' liver, extensor digitorum longus (EDL), and soleus muscle were isolated from diabetic rats treated with TAAE at a dose of 20 mg/kg at the end of the experiment to measure glycogen content using a standard method. The acute toxicity of TAAE was examined according to the OECD guideline. In addition, body weight, signs of toxicity, and/or mortality were observed for 14 days. Besides, a preliminary phytochemical screening, quantification of phenolic, flavonoid, and tannin contents as well as the antioxidant activity, were evaluated.
The results showed that TAAE at the doses of 10 and 20 mg/kg possesses a potent antihyperglycemic effect in STZ-treated diabetic rats and an acute hypoglycemic effect in normal rats, as well as the extract provoked a decrease of blood glucose levels after glucose loading in the glucose tolerance test in a dose-dependent manner. TAAE at a dose of 20 mg/kg revealed a significant improvement in the lipid profile. However, treatment with TAAE at a dose of 20 mg/kg did not significantly modify the glycogen content. In the same way, the acute toxicity analysis revealed no death or signs of toxicity in rats, and the LD50 value was more than 2 g/kg. In addition, preliminary phytochemical screening revealed that TAAE revealed the presence of polyphenols, flavonoids, tannins, carbohydrates, saponins, quinones, sterols and terpenoids. Furthermore, TAAE exhibited a potent antioxidant activity, which may be due to the richness in polyphenol content (756.21 ± 6.72 mg GAE/1 g of extract).
The current study demonstrates for the first time that aqueous Tetraclinis articulata extract has a potent glucose-lowering effect.
本研究旨在评估北非雪松的降糖效果。
北非雪松常用于治疗以慢性高血糖为特征的糖尿病。
本研究旨在评估北非雪松水提取物（TAAE）对正常及链脲佐菌素（STZ）诱导的糖尿病大鼠血糖和血脂水平的影响。此外，还评估了其急性毒性、植物化学成分及抗氧化能力。
为突出TAAE对血糖水平和脂质代谢的影响，在急性试验中，于给药后1、2、4和6小时测量血糖水平；在亚慢性试验中，于每日口服给药的第2、4和7天，以两个选定剂量（10mg/kg和20mg/kg）测量血糖水平。此外，治疗后测量甘油三酯（TGs）、总胆固醇（TC）和高密度脂蛋白胆固醇（HDL-c）。在实验结束时，从接受20mg/kg剂量TAAE治疗的糖尿病大鼠中分离肝脏、趾长伸肌（EDL）和比目鱼肌，采用标准方法测量糖原含量。根据经合组织指南检测TAAE的急性毒性。此外，观察14天的体重、毒性体征和/或死亡率。此外，还进行了初步的植物化学筛选，评估了酚类、黄酮类和单宁含量的定量以及抗氧化活性。
结果表明，10mg/kg和20mg/kg剂量的TAAE对STZ诱导的糖尿病大鼠具有显著的降血糖作用，对正常大鼠具有急性降血糖作用，并且在葡萄糖耐量试验中，提取物在葡萄糖负荷后能以剂量依赖的方式降低血糖水平。20mg/kg剂量的TAAE显示血脂水平有显著改善。然而，20mg/kg剂量的TAAE治疗并未显著改变糖原含量。同样，急性毒性分析显示大鼠无死亡或毒性迹象，LD50值大于2g/kg。此外，初步植物化学筛选显示TAAE含有多酚、黄酮类、单宁、碳水化合物、皂苷、醌类、甾醇和萜类。此外，TAAE表现出强大的抗氧化活性，这可能归因于其丰富的多酚含量（756.21±6.72mg GAE/1g提取物）。
本研究首次证明北非雪松水提取物具有强大的降糖作用。
