Folnožić I, Turk R, Đuričić D, Vince S, Pleadin J, Flegar-Meštrić Z, Valpotić H, Dobranić T, Gračner D, Samardžija M
Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Veterinary Practice Đurđevac, Đurđevac, Croatia.
Reprod Domest Anim. 2015 Dec;50(6):910-7. doi: 10.1111/rda.12608. Epub 2015 Sep 24.
The objectives of this study were to examine the influence of body condition of cows on metabolic and antioxidative status, as well as to investigate the relationship between metabolic indicators of lipid mobilization and oxidative stress during transition period. The study was conducted on 24 Holstein-Friesian dairy cows divided into 2 groups according to their body condition score (BCS) as optimal (n = 12; BCS from 3.25 to 3.75) or adipose (n = 12; BCS ≥4). Metabolic status (glucose, triglycerides, total cholesterol, HDL cholesterol, NEFA and BHB), paraoxonase-1 (PON1) and apolipoprotein A-I (ApoA-I) were analysed in sera taken on days -30, -10, -2, 0, 5, 12, 19, 26 and 60 relative to parturition. Adipose cows had significantly higher glucose concentration at parturition being significantly decreased after parturition on days 12 and 19. Total cholesterol and HDL-C concentrations were the lowest at parturition and significantly higher on days 26 and 60 after parturition in both groups of cows. Both investigated groups had significantly higher NEFA concentration from parturition until day 19 after parturition, indicating energy deficit and an increased lipid mobilization after calving. There were no significant differences in BHB concentration during transition period in both groups. No significant differences were found in PON1 activity and ApoA-I concentration during transition period in both groups of cows. However, in adipose cows, although not significantly different, PON1 was decreased from calving until day 19 after parturition indicating a disturbance in antioxidative status in adipose cows. PON1 significantly positively correlated with total cholesterol and HDL-C concentrations and negatively with NEFA indicating a strong relationship of PON1 with lipid metabolism. Significant positive correlation between NEFA and BHB in both groups of cows points out on energy deficit during transition period that cows tend to overcome by lipid mobilization providing alternative source of energy needed for parturition and lactation.
本研究的目的是考察奶牛体况对代谢和抗氧化状态的影响，以及研究围产期脂质动员的代谢指标与氧化应激之间的关系。本研究以24头荷斯坦-弗里生奶牛为对象，根据体况评分（BCS）分为两组，即最佳体况组（n = 12；BCS为3.25至3.75）和肥胖组（n = 12；BCS≥4）。在相对于分娩的第-30、-10、-2、0、5、12、19、26和60天采集血清，分析代谢状态（葡萄糖、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、非酯化脂肪酸和β-羟基丁酸）、对氧磷酶-1（PON1）和载脂蛋白A-I（ApoA-I）。肥胖奶牛在分娩时葡萄糖浓度显著较高，在分娩后第12天和第19天显著下降。两组奶牛的总胆固醇和高密度脂蛋白胆固醇浓度在分娩时最低，在分娩后第26天和第60天显著升高。从分娩到分娩后第19天，两个研究组的非酯化脂肪酸浓度均显著较高，表明能量不足以及产犊后脂质动员增加。两组在围产期β-羟基丁酸浓度上没有显著差异。两组奶牛在围产期PON1活性和ApoA-I浓度上均未发现显著差异。然而，在肥胖奶牛中，虽然差异不显著，但从产犊到分娩后第19天PON1有所下降，表明肥胖奶牛的抗氧化状态受到干扰。PON1与总胆固醇和高密度脂蛋白胆固醇浓度显著正相关，与非酯化脂肪酸显著负相关，表明PON1与脂质代谢密切相关。两组奶牛中非酯化脂肪酸和β-羟基丁酸之间的显著正相关表明围产期存在能量不足，奶牛倾向于通过脂质动员来克服，以提供分娩和泌乳所需的替代能量来源。
