Pedersen Ronnie O, Kong Jing, Achim Catalina, LaBean Thomas H
Department of Chemistry, Duke University, 124 Science Drive, Durham, NC 27708-0354, USA.
Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Molecules. 2015 Sep 23;20(9):17645-58. doi: 10.3390/molecules200917645.
DNA has shown great promise as a building material for self-assembling nanoscale structures. To further develop the potential of this technology, more methods are needed for functionalizing DNA-based nanostructures to increase their chemical diversity. Peptide nucleic acid (PNA) holds great promise for realizing this goal, as it conveniently allows for inclusion of both amino acids and peptides in nucleic acid-based structures. In this work, we explored incorporation of a positively charged PNA within DNA nanostructures. We investigated the efficiency of annealing a lysine-containing PNA probe with complementary, single-stranded DNA sequences within nanostructures, as well as the efficiency of duplex invasion and its dependence on salt concentration. Our results show that PNA allows for toehold-free strand displacement and that incorporation yield depends critically on binding site geometry. These results provide guidance for the design of PNA binding sites on nucleic acid nanostructures with an eye towards optimizing fabrication yield.
DNA作为一种用于自组装纳米级结构的建筑材料已展现出巨大潜力。为了进一步开发这项技术的潜力，需要更多方法来对基于DNA的纳米结构进行功能化，以增加其化学多样性。肽核酸（PNA）在实现这一目标方面具有很大潜力，因为它能够方便地在基于核酸的结构中同时纳入氨基酸和肽。在这项工作中，我们探索了在DNA纳米结构中引入带正电荷的PNA。我们研究了含赖氨酸的PNA探针与纳米结构内互补单链DNA序列退火的效率，以及双链侵入的效率及其对盐浓度的依赖性。我们的结果表明，PNA允许无引发链的链置换，并且掺入产率关键取决于结合位点的几何形状。这些结果为核酸纳米结构上PNA结合位点的设计提供了指导，旨在优化制造产率。
