Jo Yoon Nam, Park Byung-Dae, Um In Chul
Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
Department of Wood and Paper Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
Int J Biol Macromol. 2015 Nov;81:936-41. doi: 10.1016/j.ijbiomac.2015.09.016. Epub 2015 Sep 25.
Owing to unique properties, including the wound healing effect, sericin gel and films have attracted significant attention in the biomedical and cosmetic fields. The structural characteristics and properties of sericin gels and films are especially important owing to their effect on the performance of sericin in biomedical and cosmetic applications. In the present study, the effect of temperature on the gelation behavior, gel disruption, and sol-gel transition of sericin was examined using rheometry. In addition, the effect of the drying temperature on the structural characteristics of the sericin film was determined via Fourier transform infrared (FTIR) spectroscopy. The strength of the sericin gel increased and the gelation process was prolonged with decreasing storage temperatures. FTIR and differential scanning calorimetry (DSC) results also revealed that the crystallinity and the thermal decomposition temperature of the sericin film increased with decreasing drying temperature. The sericin gels were disrupted at a storage time of 40min when they were stored at temperatures higher than 50°C, and the corresponding gel strength decreased with increasing temperature. Furthermore, the thermo-reversible nature of gel-sol transition of sericin was confirmed by rheological and FTIR measurements.
由于具有独特的性能，包括伤口愈合效果，丝胶蛋白凝胶和薄膜在生物医学和化妆品领域引起了广泛关注。丝胶蛋白凝胶和薄膜的结构特征和性能因其对丝胶蛋白在生物医学和化妆品应用中的性能影响而尤为重要。在本研究中，使用流变学研究了温度对丝胶蛋白的凝胶化行为、凝胶破坏和溶胶-凝胶转变的影响。此外，通过傅里叶变换红外（FTIR）光谱法测定了干燥温度对丝胶蛋白薄膜结构特征的影响。随着储存温度的降低，丝胶蛋白凝胶的强度增加，凝胶化过程延长。FTIR和差示扫描量热法（DSC）结果还表明，丝胶蛋白薄膜的结晶度和热分解温度随着干燥温度的降低而增加。当丝胶蛋白凝胶在高于50°C的温度下储存时，在储存40分钟时会被破坏，并且相应的凝胶强度随温度升高而降低。此外，通过流变学和FTIR测量证实了丝胶蛋白凝胶-溶胶转变的热可逆性质。
