Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Environ Sci Pollut Res Int. 2017 Nov;24(31):24447-24453. doi: 10.1007/s11356-017-0137-5. Epub 2017 Sep 11.
Oral mucositis (OM) is one of the cancer chemotherapy-related side effects which can affect the quality of life of affected patients. This study was designed to investigate the healing effect of Elaeagnus angustifolia in 5-flurouracil (5-FU)-induced OM in golden hamster. Fifty-six adult male golden hamsters received three intraperitoneal injections of 5-FU at a dose of 60 mg/kg on days 0, 5, and 10. The cheek pouch mucosa was scratched superficially under local anesthesia. Then, two horizontal scratches were made across the everted cheek pouch on days 3 and 4. All treatments were started on day 12 for equal number of animals in control group with no treatments, gel base group that was treated with carboxy methyl cellulose as gel base which used in preparation of the topical gel, topical gel group that used gel containing 10% hydroalcoholic extract of E. angustifolia (HEEA) topically, and dietary group which was treated with 300 mg/kg HEEA. At 2 and 5 days after treatment, blood and pouch tissue sampling were done and analyzed for blood composition, tissue malondialdehyde (MDA) level, and myeloperoxidase (MPO) and superoxide dismutase (SOD) activities plus histopathological evaluations. Both topically and orally HEEA-treated groups showed a significant relief in OM compared to the control and base gel groups. However, the systemic form had higher efficiency in some parts especially decreasing the MPO (0.27 ± 0.17 vs. 0.56 ± 0.17 IU/L) and increasing SOD (6.46 ± 0.15 vs. 5.36 ± 0.18 IU/L) activities in pouch tissue in comparison to topical form mostly at 5 days after treatment. It seems that hydroalcoholic extract of E. angustifolia can be used as an appropriate drug choice for the treatment of oral mucositis based on its healing stimulatory and anti-inflammatory properties.
口腔黏膜炎（OM）是癌症化疗相关的副作用之一，会影响受影响患者的生活质量。本研究旨在探讨沙棘在 5-氟尿嘧啶（5-FU）诱导的金黄地鼠 OM 中的愈合作用。56 只成年雄性金黄地鼠在第 0、5 和 10 天接受 3 次 60mg/kg 的腹腔注射 5-FU。在局部麻醉下，颊囊黏膜被浅表划痕。然后，在第 3 和 4 天，在外翻的颊囊中制作两条水平划痕。所有治疗均于第 12 天开始，对照组无治疗，凝胶基础组用羧甲基纤维素作为凝胶基础治疗，局部用含 10%沙棘水醇提取物（HEEA）的局部凝胶治疗，饮食组用 300mg/kg 的 HEEA 治疗。治疗后 2 和 5 天，取血和囊组织进行分析，检测血液成分、组织丙二醛（MDA）水平、髓过氧化物酶（MPO）和超氧化物歧化酶（SOD）活性，并进行组织病理学评价。与对照组和基础凝胶组相比，局部和口服 HEEA 治疗组 OM 均有明显缓解。然而，全身给药形式在某些方面的效率更高，尤其是降低 MPO（0.27±0.17 对 0.56±0.17IU/L）和增加 SOD（6.46±0.15 对 5.36±0.18IU/L）活性，与局部给药形式相比，主要在治疗后 5 天。沙棘水醇提取物具有促进愈合和抗炎作用，可作为治疗口腔黏膜炎的合适药物选择。
