College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Center for Training, Consultancy and Technology Transfer, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District, Ha Noi City, Viet Nam.
Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.
Chemosphere. 2016 Feb;144:661-70. doi: 10.1016/j.chemosphere.2015.09.028. Epub 2015 Sep 25.
Nanoparticles and transgenic plants are recent scientific developments that require systematic study to understand their potential risks to human health. The effects of CuO nanoparticles (NPs) on Bt-transgenic cotton and conventional cotton are reported here. CuO NPs inhibited the growth, development, nutrient content, and indole-3-acetic acid (IAA) and abscisic acid (ABA) concentrations of transgenic and conventional cotton. Transmission electron microscopy (TEM) images showed CuO NPs aggregated on the epidermis of conventional cotton leaves, whereas it had reached into the cells of transgenic cotton leaves by endocytosis. Most CuO NPs aggregates were found on the root outer epidermis and the rest were located in intercellular spaces of both conventional and Bt-transgenic cottons. CuO NPs enhanced the expression of the exogenous gene encoding of Bt toxin protein in leaves and roots, especially at low CuO NP concentrations, providing an important benefit for Bt cotton insect resistance.
纳米粒子和转基因植物是最近的科学发展，需要系统研究才能了解它们对人类健康的潜在风险。本文报道了氧化铜纳米粒子（CuO NPs）对转 Bt 基因棉花和常规棉花的影响。CuO NPs 抑制了转 Bt 基因棉花和常规棉花的生长、发育、养分含量以及吲哚-3-乙酸（IAA）和脱落酸（ABA）的浓度。透射电子显微镜（TEM）图像显示，CuO NPs 在常规棉花叶片的表皮上聚集，而在转 Bt 基因棉花叶片中则通过内吞作用进入细胞。大多数 CuO NPs 聚集在根的外表皮上，其余的则分布在常规棉花和转 Bt 基因棉花的细胞间隙中。CuO NPs 增强了叶片和根部中外源基因编码的 Bt 毒素蛋白的表达，尤其是在低浓度的 CuO NPs 下，这为 Bt 棉花的抗虫性提供了重要的好处。
