Wong Kwan Yeung, Li Zhenhai, Zhang Xiaoqin, Leung Gilberto Ka Kit, Chan Godfrey Chi-Fung, Chim Chor Sang
Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong.
Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong.
Mol Cancer. 2015 Sep 26;14:175. doi: 10.1186/s12943-015-0444-8.
In multiple myeloma, a long non-coding RNA, KIAA0495 (alias PDAM/TP73-AS1), had been found progressively downregulated from normal plasma cell to benign monoclonal gammopathy of undetermined significance to symptomatic myeloma. Herein, by methylation-specific PCR, the putative KIAA0495 promoter was found unmethylated in all healthy controls (N = 14) but methylated in 50 % of myeloma cell lines (N = 10). KIAA0495 methylation was shown inversely correlated with KIAA0495 expression. However, KIAA0495 methylation was detected in none of both primary myeloma samples at diagnosis (N = 61) and at relapse/progression (N = 16). Collectively, despite frequently methylated in cell lines, KIAA0495 methylation appeared unimportant in the pathogenesis or progression of myeloma.
在多发性骨髓瘤中，一种长链非编码RNA，即KIAA0495（别名PDAM/TP73-AS1），已被发现从正常浆细胞到意义未明的良性单克隆丙种球蛋白病再到有症状的骨髓瘤呈现逐渐下调的趋势。在此，通过甲基化特异性PCR发现，假定的KIAA0495启动子在所有健康对照（N = 14）中未甲基化，但在50%的骨髓瘤细胞系（N = 10）中甲基化。结果显示，KIAA0495甲基化与KIAA0495表达呈负相关。然而，在诊断时的原发性骨髓瘤样本（N = 61）和复发/进展期的原发性骨髓瘤样本（N = 16）中均未检测到KIAA0495甲基化。总体而言，尽管KIAA0495在细胞系中经常发生甲基化，但KIAA0495甲基化在骨髓瘤的发病机制或进展中似乎并不重要。
