74648Department of Medical Oncology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
74648Department of Gastroenterology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820945770. doi: 10.1177/1533033820945770.
Long noncoding RNAs (lncRNAs) are potential biomarkers for cancers. Nevertheless, the ability of long noncoding RNA lung cancer-associated transcript 1 in patients with multiple myeloma remains unknown. The purpose of this current study was to figure out its function in multiple myeloma.
Firstly, the expression of long noncoding RNA lung cancer-associated transcript 1 in cancer or normal tissues and serum from patients with multiple myeloma and normal donors was detected. Secondly, the expression of long noncoding RNA lung cancer-associated transcript 1 was overexpressed or silenced in U266 and H929 cells, respectively to detect changes of proliferation and apoptosis in multiple myeloma . Subsequently, the expression of transforming growth factor-β signaling pathway-related proteins was detected by western blot analysis. Finally, the effect of long noncoding RNA lung cancer-associated transcript 1 on the growth of multiple myeloma cells was evaluated by tumor xenograft in nude mice.
Long noncoding RNA lung cancer-associated transcript 1 was increased in cancer tissues and serum of patients with multiple myeloma as well as multiple myeloma cells, which was correlated with dismal prognosis of patients with multiple myeloma. Overexpression of long noncoding RNA lung cancer-associated transcript 1 promoted the activity of U266 and H929 cells, while inhibition of long noncoding RNA lung cancer-associated transcript 1 suppressed the activity of U266 and H929 cells. In addition, long noncoding RNA lung cancer-associated transcript 1 was found to promote activation of the transforming growth factor-β signaling pathway. Furthermore, long noncoding RNA lung cancer-associated transcript 1 knockdown restricted the growth of multiple myeloma cells .
This study suggests that suppression of long noncoding RNA lung cancer-associated transcript 1 inhibits the activation of transforming growth factor-β signaling pathway, thereby inhibiting the growth of multiple myeloma cells.
长链非编码 RNA（lncRNA）是癌症的潜在生物标志物。然而，长链非编码 RNA 肺癌相关转录本 1 在多发性骨髓瘤患者中的能力尚不清楚。本研究旨在探讨其在多发性骨髓瘤中的作用。
首先，检测了癌症或正常组织以及多发性骨髓瘤患者和正常供体血清中的长链非编码 RNA 肺癌相关转录本 1 的表达。其次，分别在 U266 和 H929 细胞中转染长链非编码 RNA 肺癌相关转录本 1 过表达或沉默，检测多发性骨髓瘤细胞增殖和凋亡的变化。随后，通过 Western blot 分析检测转化生长因子-β信号通路相关蛋白的表达。最后，通过裸鼠肿瘤异种移植评估长链非编码 RNA 肺癌相关转录本 1 对多发性骨髓瘤细胞生长的影响。
长链非编码 RNA 肺癌相关转录本 1 在多发性骨髓瘤患者的癌组织和血清以及多发性骨髓瘤细胞中均增加，与多发性骨髓瘤患者的预后不良相关。长链非编码 RNA 肺癌相关转录本 1 的过表达促进了 U266 和 H929 细胞的活性，而长链非编码 RNA 肺癌相关转录本 1 的抑制则抑制了 U266 和 H929 细胞的活性。此外，发现长链非编码 RNA 肺癌相关转录本 1 促进了转化生长因子-β信号通路的激活。此外，长链非编码 RNA 肺癌相关转录本 1 敲低限制了多发性骨髓瘤细胞的生长。
本研究表明，抑制长链非编码 RNA 肺癌相关转录本 1 抑制了转化生长因子-β信号通路的激活，从而抑制了多发性骨髓瘤细胞的生长。
