从空调系统冷却水中分离出的弗朗西斯菌属的特性分析。
Characterization of Francisella species isolated from the cooling water of an air conditioning system.
作者信息
Gu Quan, Li Xunde, Qu Pinghua, Hou Shuiping, Li Juntao, Atwill Edward R, Chen Shouyi
机构信息
Guangzhou Center for Disease Control and Prevention, Guangzhou, China .
Department of Population Health and Reproduction, University of California, California, USA .
出版信息
Braz J Microbiol. 2015 Jul 1;46(3):921-7. doi: 10.1590/S1517-838246320140465. eCollection 2015 Jul-Sep.
Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems.
从中国广州一个空调系统的冷却水中分离出了弗朗西斯菌属菌株。这些菌株为革兰氏阴性、球杆菌、无动力、氧化酶阴性、过氧化氢酶阴性、酯酶和脂质酯酶阳性。此外，这些细菌在添加半胱氨酸的培养基上于20℃至40℃生长，最适生长温度为30℃。16S rRNA基因序列分析表明这些菌株属于弗朗西斯菌属。16S rRNA、rpoB和sdhA基因的生化试验以及系统发育和BLAST分析表明，一株菌株与嗜肺弗朗西斯菌非常相似，其他菌株与我们之前描述的广州弗朗西斯菌新种菌株08HL01032相同或高度相似。这些菌株的生化和分子特征表明空调系统中存在多种弗朗西斯菌属物种。
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