Lee Hyung Chul, Choi Hee Jung, Lee Hyo Gun, Lim Jeong Mook, Ono Tamao, Han Jae Yong
1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.
2 Department of Cell and Developmental Biology, University College London , London, United Kingdom .
Stem Cells Dev. 2016 Jan 1;25(1):68-79. doi: 10.1089/scd.2015.0208. Epub 2015 Oct 27.
The timing and biological events associated with germ cell specification in chickens have not been determined yet. In this study, we report the origin of primordial germ cells (PGCs) and germ plasm dynamics through investigation of the expression of the chicken homolog of deleted in azoospermia-like (cDAZL) gene during germ cell specification. Asymmetric localization of germ plasm in the center of oocytes from preovulatory follicle stages leads to PGCs being formed in the center. During cleavage stages, DAZL expression pattern changes from a subcellular localization to a diffuse form before and after zygotic genome activation. Meanwhile, PGCs exhibit transcriptional active status during their specification. In addition, knockdown studies of cDAZL, which result in reduced proliferation, aberrant gene expression profiles, and PGC apoptosis in vitro, suggest its possible roles for PGC formation in chicken. In conclusion, DAZL expression reveals formation and initial positioning of PGCs in chickens.
鸡中与生殖细胞特化相关的时间和生物学事件尚未确定。在本研究中，我们通过研究无精子症样缺失基因的鸡同源物（cDAZL）基因在生殖细胞特化过程中的表达，报告了原始生殖细胞（PGC）的起源和生殖质动态。排卵前卵泡阶段卵母细胞中心生殖质的不对称定位导致PGC在中心形成。在卵裂阶段，DAZL的表达模式在合子基因组激活前后从亚细胞定位变为弥散形式。同时，PGC在其特化过程中表现出转录活跃状态。此外，对cDAZL的敲低研究导致体外增殖减少、基因表达谱异常和PGC凋亡，提示其在鸡PGC形成中的可能作用。总之，DAZL表达揭示了鸡中PGC的形成和初始定位。
