Friedrich-Loeffler-Institut, Institute of Farm Animal Genetics, Department of Biotechnology, Stem Cell Unit, Mariensee, Höltystr. 10, 31535, Neustadt, Germany.
Institut Für Humangenetik, Department of Developmental Biochemistry, Georg-August-Universität Göttingen, 37077, Göttingen, Germany.
Sci Rep. 2024 Nov 12;14(1):27753. doi: 10.1038/s41598-024-78544-7.
Maternal germ plasm determines the germline in birds. Previously, we proposed the chicken-specific Bucky ball (cBuc) as a functional equivalent of the zebrafish germ plasm organizer. This study demonstrated the maternal cBuc synthesis, and verified a highly dynamic distribution of Bucky ball from oocyte nests to maturing follicles using specific antibodies. The dynamic re-localization of cBuc from the ovarian stroma to the granulosa cells, and the Balbiani structure of the oocyte was revealed. Following the accumulation of cBuc in the Balbiani body, an increased signal of chicken vasa homolog (CVH) in close contact to cBuc could be detected. Highest transcription of cBuc was recorded in follicles with diameters up to 500 µm. First RNA-interference experiments in an in-vivo follicle culture assay revealed inhibiting effects on cBuc in small follicles. These data demonstrate the maternal origin of cBuc, and underpin its role as germ plasm organizer.
母系生殖质决定鸟类的生殖系。此前，我们提出鸡特异性巴基球（cBuc）是斑马鱼生殖质组织者的功能等同物。本研究证明了母系 cBuc 的合成，并使用特异性抗体验证了巴基球从卵母细胞巢到成熟滤泡的高度动态分布。cBuc 从卵巢基质到颗粒细胞的动态重新定位，以及卵母细胞的巴尔比尼结构被揭示。cBuc 在巴尔比尼体中积累后，可以检测到与 cBuc 紧密接触的鸡 vasa 同源物（CVH）信号增加。cBuc 的转录水平在直径达 500µm 的滤泡中最高。在体内滤泡培养实验中的首次 RNA 干扰实验显示，在小滤泡中对 cBuc 具有抑制作用。这些数据证明了 cBuc 的母系起源，并支持其作为生殖质组织者的作用。
