Liu Ran-ran, Yang Zhi-hong, Yang Yu-ling, Liu Zhan-tao, Yue Wang
Zhong Yao Cai. 2015 Feb;38(2):339-43.
To study the antiemetic effect of Ju-Pi-Tang from Jin Kui Yao Lue on cisplatin-induced emetic model in minks, and to observe the immunoexpression of peripheral and central c-fos and substance P.
The minks were randomly divided into blank control group, Ju-Pi-Tang blank control group, model group, ondansetron group, aprepitant group, Ju-Pi-Tang (in high-, mid-, and low-dose) groups. Every group was administered with the antiemetic agent or distilled water on 24 h before cisplatin injection. The antiemetic effect of drugs was investigated in the emetic model of minks induced by cisplatin in 72 h observation. Immunohistochemistry was used to compare the differences of c-fos and substance P expression in the area postrema of brain and distal ileum tissues.
During observation period,compared with model group,the frequency cisplatin induced retching and vomiting was significantly reduced by Ju-Pi-Tang in high- and mid-dose groups, during the 0-24 h acute period, the number of retching of Ju-Pi-Tang in high-dose group was decreased more than aprepitant group, during the 24-72 h delayed period, the number of both retching and vomiting was decreased more than ondansetron group, after 72 h of cisplatin administration, compared with model group, the grey levels of c-fos and substance P expression in distal ileum and brain tissues of Ju-Pi-Tang groups were higher significantly.
Ju-Pi-Tang has a good effect against cisplatin-induced emesis in minks.
研究《金匮要略》橘皮汤对顺铂致水貂呕吐模型的止吐作用，并观察外周及中枢c-fos和P物质的免疫表达。
将水貂随机分为空白对照组、橘皮汤空白对照组、模型组、昂丹司琼组、阿瑞匹坦组、橘皮汤高、中、低剂量组。每组在顺铂注射前24 h给予止吐药或蒸馏水。在72 h观察期内，观察顺铂致水貂呕吐模型中药物的止吐效果。采用免疫组织化学法比较脑最后区和回肠末端组织中c-fos和P物质表达的差异。
观察期内，与模型组比较，橘皮汤高、中剂量组可显著减少顺铂致干呕和呕吐的次数，在0-24 h急性期，橘皮汤高剂量组干呕次数减少较阿瑞匹坦组多，在24-72 h延迟期，干呕和呕吐次数减少较昂丹司琼组多，顺铂给药72 h后，与模型组比较，橘皮汤各组回肠末端和脑组织中c-fos和P物质表达的灰度值显著升高。
橘皮汤对顺铂致水貂呕吐有良好的防治作用。
