Zhang Fang, Wang Lei, Yang Zhi-Hong, Liu Zhan-Tao, Yue Wang
Department of Pharmacology, Medical College of Qingdao University, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China.
World J Gastroenterol. 2006 Feb 28;12(8):1300-2. doi: 10.3748/wjg.v12.i8.1300.
To establish a new, reliable vomit model of minks.
Adult male minks were randomly divided into 8 groups (n=6): cisplatin (7.5 mg/kg) intraperitoneal injection (i.p.) group, copper sulfate (40 mg/kg) intragastric injection (i.g.) group, apomorphine (1.6 mg/kg) subcutaneous injection (s.c.) group, and 18 Gy whole-body X-irradiation group, ondansetron injection group (2 mg/kg i.p.) 30 min later followed by cisplatin (7.5 mg/kg) i.p., normal saline (NS) i.p. injection control group, metoclopramide injection group (4 mg/kg i.p.) 30 min later followed by apomorphine (1.6 mg/kg) s.c., NS i.g. control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method.
Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups (P=0.018). Immunohistologic result showed that 5-HT released from enterochromaffin cells (EC cells) was involved in vomiting mechanism.
Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.
建立一种新的、可靠的水貂呕吐模型。
将成年雄性水貂随机分为8组（n = 6）：顺铂（7.5 mg/kg）腹腔注射组、硫酸铜（40 mg/kg）灌胃组、阿扑吗啡（1.6 mg/kg）皮下注射组、18 Gy全身X射线照射组、昂丹司琼注射组（2 mg/kg腹腔注射），30分钟后腹腔注射顺铂（7.5 mg/kg）、生理盐水腹腔注射对照组、甲氧氯普胺注射组（4 mg/kg腹腔注射），30分钟后皮下注射阿扑吗啡（1.6 mg/kg）、生理盐水灌胃对照组。计算干呕和呕吐的频率。行为实验后，采用免疫组织学方法检测回肠中5-羟色胺（5-HT）的分布。
给貂注射顺铂、阿扑吗啡、硫酸铜和进行X射线照射后，动物出现强烈的呕吐反应。然而，在顺铂和硫酸铜组中，昂丹司琼和甲氧氯普胺预处理可显著抑制干呕和呕吐（P = 0.018）。免疫组织学结果表明，肠嗜铬细胞（EC细胞）释放的5-HT参与呕吐机制。
水貂呕吐模型在研究呕吐机制和筛选新型止吐药物方面具有重要价值。
