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基于基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的产超广谱β-内酰胺酶大肠杆菌分型——实时暴发调查的新工具

Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation.

作者信息

Egli Adrian, Tschudin-Sutter Sarah, Oberle Michael, Goldenberger Daniel, Frei Reno, Widmer Andreas F

机构信息

Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Infection Biology Lab, Department Biomedicine, University of Basel, Basel, Switzerland.

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.

出版信息

PLoS One. 2015 Apr 10;10(4):e0120624. doi: 10.1371/journal.pone.0120624. eCollection 2015.

DOI:10.1371/journal.pone.0120624
PMID:25860943
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4393243/
Abstract

Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spectrometry based typing that confirmed relatedness faster and more cost-effective, but as reliable as PFGE.

摘要

通过脉冲场凝胶电泳（PFGE）等分子分型方法对菌株进行分型，从而确认存在流行病学关联的聚集性病例。我们将一个PFGE相关聚集性病例中的6株产超广谱β-内酰胺酶大肠杆菌与基于基质辅助激光解吸/电离飞行时间质谱的分型方法进行了比较，该方法确认相关性的速度更快、成本效益更高，且与PFGE一样可靠。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b25/4393243/6424bce66dee/pone.0120624.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b25/4393243/2a8e936392d5/pone.0120624.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b25/4393243/6424bce66dee/pone.0120624.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b25/4393243/2a8e936392d5/pone.0120624.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b25/4393243/6424bce66dee/pone.0120624.g002.jpg

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