4-辛基苯酚对鱼类肝细胞的细胞毒性作用。
Cytotoxic effects of 4-octylphenol on fish hepatocytes.
作者信息
Kaptaner Burak
机构信息
Department of Biology, Faculty of Science, Yüzüncü Yıl University, 65080, Van, Turkey.
出版信息
Cytotechnology. 2016 Aug;68(4):1577-83. doi: 10.1007/s10616-015-9916-3. Epub 2015 Sep 28.
Abstract
The present study was conducted to determine cytotoxic effects of 4-octylphenol (4-OP) on primary cultured hepatocytes of pearl mullet (Alburnus tarichi). Lactate dehydrogenase (LDH) release, malondialdehyde (MDA) level, antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST)] and glutathione (GSH) content were measured after 24-h exposure to 4-OP. 4-OP caused dose- and time-dependent increases in LDH release. Significant induction of MDA level and decrease in GSH content were found. SOD and GPx activities were decreased while GST activity was increased. These findings suggest that 4-OP leads to cytotoxicity by depressing antioxidant defenses in fish hepatocytes.
摘要
本研究旨在确定4-辛基酚（4-OP）对珍珠鲴（Alburnus tarichi）原代培养肝细胞的细胞毒性作用。在暴露于4-OP 24小时后，测定乳酸脱氢酶（LDH）释放、丙二醛（MDA）水平、抗氧化酶[超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）]以及谷胱甘肽（GSH）含量。4-OP导致LDH释放呈剂量和时间依赖性增加。发现MDA水平显著升高而GSH含量降低。SOD和GPx活性降低，而GST活性增加。这些发现表明，4-OP通过抑制鱼类肝细胞中的抗氧化防御导致细胞毒性。
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