Takhtfooladi Mohammad Ashrafzadeh, Sharifi Davood
Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Lasers Med Sci. 2015 Dec;30(9):2319-24. doi: 10.1007/s10103-015-1813-7. Epub 2015 Sep 28.
This study aimed at evaluating the effects of red and blue light-emitting diodes (LED) and low-level laser (LLL) on the regeneration of the transected sciatic nerve after an end-to-end neurorrhaphy in rabbits. Forty healthy mature male New Zealand rabbits were randomly assigned into four experimental groups: control, LLL (680 nm), red LED (650 nm), and blue LED (450 nm). All animals underwent the right sciatic nerve neurotmesis injury under general anesthesia and end-to-end anastomosis. The phototherapy was initiated on the first postoperative day and lasted for 14 consecutive days at the same time of the day. On the 30th day post-surgery, the animals whose sciatic nerves were harvested for histopathological analysis were euthanized. The nerves were analyzed and quantified the following findings: Schwann cells, large myelinic axons, and neurons. In the LLL group, as compared to other groups, an increase in the number of all analyzed aspects was observed with significance level (P < 0.05). This finding suggests that postoperative LLL irradiation was able to accelerate and potentialize the peripheral nerve regeneration process in rabbits within 14 days of irradiation.
本研究旨在评估红色和蓝色发光二极管（LED）及低强度激光（LLL）对兔坐骨神经端端神经缝合术后横断坐骨神经再生的影响。40只健康成熟雄性新西兰兔被随机分为四个实验组：对照组、LLL（680纳米）组、红色LED（650纳米）组和蓝色LED（450纳米）组。所有动物均在全身麻醉下接受右侧坐骨神经切断伤并进行端端吻合。术后第一天开始光疗，每天在同一时间持续进行14天。术后第30天，对坐骨神经进行取材用于组织病理学分析的动物实施安乐死。对神经进行分析并量化以下指标：雪旺细胞、有髓大轴突和神经元。在LLL组中，与其他组相比，所有分析指标的数量均有增加，差异有统计学意义（P < 0.05）。这一发现表明，术后LLL照射能够在照射14天内加速并促进兔外周神经再生过程。
