Zayit-Soudry Shiri, Sippl-Swezey Nicolas, Porco Travis C, Lynch Stephanie K, Syed Reema, Ratnam Kavitha, Menghini Moreno, Roorda Austin J, Duncan Jacque L
Department of Ophthalmology University of California, San Francisco, California, United States.
Department of Ophthalmology University of California, San Francisco, California, United States 2Francis I. Proctor Foundation, Department of Ophthalmology, University of California, San Francisco, California, United States.
Invest Ophthalmol Vis Sci. 2015 Sep 1;56(10):6179-89. doi: 10.1167/iovs.15-17010.
To determine short-term variability of adaptive optics scanning laser ophthalmoscopy (AOSLO)-derived cone spacing measures in eyes with inherited retinal degenerations (IRD) and in normal eyes.
Twenty IRD patients and 10 visually normal subjects underwent AOSLO imaging at two visits separated by no more than 1 month (NCT00254605). Cone spacing was measured in multiple macular regions in each image by three independent graders. Variability of cone spacing measures between visits, between graders, and between eyes was determined and correlated with standard clinical measures.
Cone spacing was measured in 2905 regions. Interobserver agreement was high both in normal eyes and eyes with IRD (mean intraclass correlation coefficient [ICC] = 0.838 for normal and 0.892 for eyes with IRD). Cone spacing measures were closely correlated between visits (ICC > 0.869 for both study groups). Mean relative intervisit spacing difference (absolute difference in measures divided by the mean at each region) was 4.0% for normal eyes and 4.9% for eyes with IRD. Cone spacing measures from fellow eyes of the same subject showed strong agreement for all subjects (ICC > 0.85 for both study groups).
Adaptive optics scanning laser ophthalmoscopy-derived macular cone spacing measures were correlated between observers, visits, and fellow eyes of the same subject in normal eyes and in eyes with IRD. This information may help establish the role of cone spacing measures derived from images of the cone mosaic obtained with AOSLO as a sensitive biomarker for longitudinal tracking of photoreceptor loss during disease progression and in response to treatment. (ClinicalTrials.gov number, NCT00254605.).
确定遗传性视网膜变性（IRD）患者和正常眼睛中自适应光学扫描激光检眼镜（AOSLO）得出的视锥细胞间距测量值的短期变异性。
20名IRD患者和10名视力正常的受试者在两次间隔不超过1个月的检查中接受了AOSLO成像（NCT00254605）。由三名独立的评分者在每个图像的多个黄斑区域测量视锥细胞间距。确定了两次检查之间、评分者之间以及双眼之间视锥细胞间距测量值的变异性，并将其与标准临床测量值相关联。
在2905个区域测量了视锥细胞间距。正常眼睛和IRD眼睛的观察者间一致性都很高（正常眼睛的平均组内相关系数[ICC]=0.838，IRD眼睛的为0.892）。两次检查之间视锥细胞间距测量值密切相关（两个研究组的ICC均>0.869）。正常眼睛的平均相对检查间间距差异（测量值的绝对差异除以每个区域的平均值）为4.0%，IRD眼睛的为4.9%。同一受试者对侧眼的视锥细胞间距测量值在所有受试者中都显示出高度一致性（两个研究组的ICC均>0.85）。
在正常眼睛和IRD眼睛中，观察者之间、两次检查之间以及同一受试者的对侧眼之间，自适应光学扫描激光检眼镜得出的黄斑视锥细胞间距测量值具有相关性。这些信息可能有助于确定从AOSLO获得的视锥细胞镶嵌图像得出的视锥细胞间距测量值作为疾病进展和治疗反应期间光感受器损失纵向追踪的敏感生物标志物的作用。（ClinicalTrials.gov编号，NCT00254605。）
