Giannini Daniela, Lombardo Giuseppe, Mariotti Letizia, Devaney Nicholas, Serrao Sebastiano, Lombardo Marco
Fondazione G.B. Bietti IRCCS, Rome, Italy.
Consiglio Nazionale delle Ricerche, Istituto per i Processi Chimico-Fisici, Messina, Italy 3Vision Engineering Italy srl, Rome, Italy.
Invest Ophthalmol Vis Sci. 2017 Jun 1;58(7):3127-3137. doi: 10.1167/iovs.16-20890.
To assess reliability and agreement among three metrics used to evaluate the distribution of cell distances in adaptive optics (AO) images of the cone mosaic.
Using an AO flood illumination retinal camera, we acquired images of the cone mosaic in 20 healthy subjects and 12 patients with retinal diseases. The three spacing metrics studied were the center-to-center spacing (Scc), the local cone spacing (LCS), and the density recovery profile distance (DRPD). Each metric was calculated in sampling areas of different sizes (64 × 64 μm and 204 × 204 μm) across the parafovea.
Both Scc and LCS were able to discriminate between healthy subjects and patients with retinal diseases; DRPD did not reliably detect any abnormality in the distribution of cell distances in patients with retinal diseases. The agreement between Scc and LCS was high in healthy subjects (intraclass correlation coefficient [ICC] ≥ 0.79) and moderate in patients with retinal diseases (ICC ≤ 0.51). The DRPD had poor agreement with Scc (ICC ≤ 0.47) and LCS (ICC ≤ 0.37). The correlation between the spacing metrics of the two sampling areas was greater in healthy subjects than in patients with retinal diseases.
The Scc and LCS provided interchangeable estimates of cone distance in AO retinal images of healthy subjects but could not be used interchangeably when investigating retinal diseases with significant cell reflectivity loss (≥30%). The DRPD was unreliable for describing cell distance in a human retinal cone mosaic and did not correlate with Scc and LCS. Caution is needed when comparing spacing metrics evaluated in sampling areas of different sizes.
评估用于评估自适应光学（AO）视锥细胞镶嵌图像中细胞间距分布的三种指标之间的可靠性和一致性。
使用AO泛光照明视网膜相机，我们采集了20名健康受试者和12名视网膜疾病患者的视锥细胞镶嵌图像。研究的三种间距指标分别是中心到中心间距（Scc）、局部视锥细胞间距（LCS）和密度恢复轮廓距离（DRPD）。在整个旁中央凹的不同大小（64×64μm和204×204μm）的采样区域中计算每个指标。
Scc和LCS都能够区分健康受试者和视网膜疾病患者；DRPD不能可靠地检测出视网膜疾病患者细胞间距分布的任何异常。Scc和LCS在健康受试者中的一致性较高（组内相关系数[ICC]≥0.79），在视网膜疾病患者中为中等（ICC≤0.51）。DRPD与Scc（ICC≤0.47）和LCS（ICC≤0.37）的一致性较差。两个采样区域的间距指标之间的相关性在健康受试者中比在视网膜疾病患者中更大。
Scc和LCS在健康受试者的AO视网膜图像中对视锥细胞间距提供了可互换的估计，但在研究具有显著细胞反射率损失（≥30%）的视网膜疾病时不能互换使用。DRPD在描述人类视网膜视锥细胞镶嵌中的细胞间距时不可靠，并且与Scc和LCS不相关。在比较不同大小采样区域中评估的间距指标时需要谨慎。
