Zhu Yichun, Zheng Minghuan, Song Dongli, Ye Ling, Wang Xiangdong
Zhongshan Hospital, Shanghai Institute of Clinical Bioinformatics, Fudan University Center for Bioinformatics, Fudan University, Shanghai, China.
J Transl Med. 2015 Sep 28;13:318. doi: 10.1186/s12967-015-0669-8.
Telocytes (TCs) are suggested as a new type of interstitial cells with specific telopodes. Our previous study evidenced that TCs differed from fibroblasts and stem cells at the aspect of gene expression profiles. The present study aims to search the characters and patterns of chromosome X genes of TC-specific or TC-dominated gene profiles and fingerprints, investigate the network of principle genes, and explore potential functional association.
We compared gene expression profiles in chromosome X of pulmonary TCs with mesenchymal stem cells (MSC), fibroblasts (Fb), alveolar type II cells (ATII), airway basal cells (ABC), proximal airway cells (PAC), CD8(+) T cells come from bronchial lymph nodes (T-BL), or CD8(+) T cells from lungs (T-L) by global analyses, and selected the genes which were consistently up or down regulated (>1 fold) in TCs compared to other cells as TC-specific genes. The functional and characteristic networks were identified and compared by bioinformatics tools.
We selected 31 chromosome X genes as the TC-specific or dominated genes, among which 8 up-regulated (Flna, Msn, Cfp, Col4a5, Mum1l1, Rnf128, Syn1, and Srpx2) and 23 down-regulated (Abcb7, Atf1, Ddx26b, Drp2, Fam122b, Gyk, Irak1, Lamp2, Mecp2, Ndufb11, Ogt, Pdha1, Pola1, Rab9, Rbmx2, Rhox9, Thoc2, Vbp1, Dkc1, Nkrf, Piga, Tmlhe and Tsr2), as compared with other cells.
Our data suggested that gene expressions of chromosome X in TCs are different with those in other cells in the lung tissue. According to the selected TC-specific genes, we infer that pulmonary TCs function as modulators which may enhance cellular growth and migration, resist senescence, protect cells from external stress, regulate immune responses, participate in tissue remodeling and repair, regulate neural function, and promote vessel formation.
端粒细胞（TCs）被认为是一种具有特殊端粒的新型间质细胞。我们之前的研究证明，端粒细胞在基因表达谱方面与成纤维细胞和干细胞不同。本研究旨在探寻端粒细胞特异性或端粒细胞主导的基因谱和指纹图谱中X染色体基因的特征和模式，研究主要基因的网络，并探索潜在的功能关联。
我们通过全面分析，比较了肺端粒细胞与间充质干细胞（MSC）、成纤维细胞（Fb）、II型肺泡细胞（ATII）、气道基底细胞（ABC）、近端气道细胞（PAC）、来自支气管淋巴结的CD8(+)T细胞（T-BL）或来自肺的CD8(+)T细胞（T-L）的X染色体基因表达谱，并选择与其他细胞相比在端粒细胞中持续上调或下调（>1倍）的基因作为端粒细胞特异性基因。通过生物信息学工具识别并比较功能和特征网络。
我们选择了31个X染色体基因作为端粒细胞特异性或主导基因，其中8个上调（Flna、Msn、Cfp、Col4a5、Mum1l1、Rnf128、Syn1和Srpx2），23个下调（Abcb7、Atf1、Ddx26b、Drp2、Fam122b、Gyk、Irak1、Lamp2、Mecp2、Ndufb11、Ogt、Pdha1、Pola1、Rab9、Rbmx2、Rhox9、Thoc2、Vbp1、Dkc1、Nkrf、Piga、Tmlhe和Tsr2），与其他细胞相比。
我们的数据表明，端粒细胞中X染色体的基因表达与肺组织中的其他细胞不同。根据所选的端粒细胞特异性基因，我们推断肺端粒细胞起调节作用，可能增强细胞生长和迁移、抵抗衰老、保护细胞免受外部应激、调节免疫反应、参与组织重塑和修复、调节神经功能并促进血管形成。
